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DNAFingerprintLab - CHEM 4491 Lab#7 Dr Burke Spring 2011...

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CHEM 4491 Lab #7 – Dr. Burke Spring 2011 DNA Fingerprinting Crime Scene Scenario: Investigators were called to the scene of a burglary where it appeared that as the burglar rushed to leave, he ran into a glass door, cutting his arm and tearing his shirt. The investigators removed small pieces of bloodstained fabric from the door to be tested; the blood sample was determined to be type A. Two suspects were apprehended; unfortunately, both had type A blood. Investigators have now resorted to DNA fingerprinting to determine which of the two suspects is the burglar. Loading and Running a DNA Gel 1. You and a partner will be sharing a gel. Seal the edges of the gel casting tray with the rubber dams and position the gel comb in the top notch of the gel tray. 2. Prepare a 0.8% agarose gel. Weigh out 0.32 g of agarose on a balance and add 40 mL of 1 X TAE buffer to the powdered agarose in an Erlenmeyer flask to prepare a 0.8% agarose gel. Heat the solution in on a hot plate to dissolve the agarose. Cool the solution for ~2 minutes and gently pour the solution into the tray. Make sure that no bubbles are trapped underneath the comb. 3. Once the gel has cooled and set, add 1 X TAE running buffer to the gel chamber until the buffer level is 2-3 mm above the top of the gel.
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