Radial immunopercipitation

Radial immunopercipitation - Single diffusionRadial...

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SINGLE VS. DOUBLE Single diffusion Radial Supporting medium (gel) contains one reactant at a uniform concentration Only the unknowns move through the medium Double diffusion Ouchterlony Gel is inert (contains no reactants) Both Ag and Ab travel through the medium
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RADIAL IMMUNODIFFUSION Fahey method (kinetic) Read at 18 hours Plot [std] vs. ring diameter on semi-log paper Mancini method (endpoint) Read at 48 or 72 hours Plot[std] vs. ring diameter squared on graph paper Results reliable only if the ring size is within the range of the standards; if greater than highest std, dilute and repeat test
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RADIAL IMMUNODIFFUSION Ab uniformly distributed in gel; Ag diffuses outward from a well (single diffusion) AgAb complexes form as concentric
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Radial immunopercipitation - Single diffusionRadial...

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