Ouchterlony - Advantages of Ouchterlony Test 1. See more...

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Advantages of Ouchterlony Test 1. See more than 1 Ag 2. Qualitative test 3. Can identify Ags a. line of identity b. nonidentity c. partial identity Problems 1. Time Reaction occurs within this time frame 24- 48 hours 2. Sensitivity Depends on if Ab/Ag is in: Antibody-excess zone, Equivalence zone or Antigen-excess zone Ouchterlony Immunodiffusion. The method of double diffusion in a gel matrix, usually agarose, and the precipitation of antigens and antibodies that are loaded into these wells. Single Radial Immunodiffusion (Mancini Test). Is where either Ag or Ab is incorporated into gel v.s. Affinity : Strength of interaction between epitope and one antigen-binding site Avidity : Strength of the sum of interactions between antibody and antigen It is important not to place the wells too far apart, otherwise you will not get the desired precipitation.
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Antibody well Antigen, radial diffusion across concentration gradient Antibody, radial diffusion across concentration gradient
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This note was uploaded on 04/10/2011 for the course BIOL 4202 taught by Professor Staff during the Spring '11 term at North Texas.

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Ouchterlony - Advantages of Ouchterlony Test 1. See more...

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