Unformatted text preview: same plane of focus. It is important not to allow the sample to settle too long or it will dry out. Quadrants: Count as shown in the diagram: • Cell density (cells/ml)= (Total # of cells counted / 8 * (#of squares in the hemacytometer)) x 10 4 (volume factor in ml). • Total cell count= Cell density(cells/ml) x Volume of original cell suspension(ml) = Total cell count(cells) * This # could be 2, 4, or 8, it all depends on the number of square sections you count. This # represents of obtaining an average cell count between squares....
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This note was uploaded on 04/10/2011 for the course BIOL 4202 taught by Professor Staff during the Spring '11 term at North Texas.
- Spring '11