using the Hemacytometer

using the Hemacytometer - same plane of focus It is...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Hemacytometer: Loading the hemacytometer. Place the coverslip over the counting chambers. Load both counting chambers with the cell suspension using a micropipette and tip, or a Pasteur pippete. Approximately 10ul will be required per side (chamber). Place the pipette tip at the edge of the coverslip in the V-shaped groove, and allow the cell suspension to fill the space by capillary action. Fill the entire volume of the chamber, but do not overfill . If suspension overflows into the next set of 9 quadrants, then you will have to start again. The sample is allowed to settle for 2 or 3 minutes so that the cells stop drifting around the chamber and most will be in the
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: same plane of focus. It is important not to allow the sample to settle too long or it will dry out. Quadrants: Count as shown in the diagram: • Cell density (cells/ml)= (Total # of cells counted / 8 * (#of squares in the hemacytometer)) x 10 4 (volume factor in ml). • Total cell count= Cell density(cells/ml) x Volume of original cell suspension(ml) = Total cell count(cells) * This # could be 2, 4, or 8, it all depends on the number of square sections you count. This # represents of obtaining an average cell count between squares....
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern