using the Hemacytometer

using the Hemacytometer - same plane of focus It is...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Hemacytometer: Loading the hemacytometer. Place the coverslip over the counting chambers. Load both counting chambers with the cell suspension using a micropipette and tip, or a Pasteur pippete. Approximately 10ul will be required per side (chamber). Place the pipette tip at the edge of the coverslip in the V-shaped groove, and allow the cell suspension to fill the space by capillary action. Fill the entire volume of the chamber, but do not overfill . If suspension overflows into the next set of 9 quadrants, then you will have to start again. The sample is allowed to settle for 2 or 3 minutes so that the cells stop drifting around the chamber and most will be in the
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: same plane of focus. It is important not to allow the sample to settle too long or it will dry out. Quadrants: Count as shown in the diagram: • Cell density (cells/ml)= (Total # of cells counted / 8 * (#of squares in the hemacytometer)) x 10 4 (volume factor in ml). • Total cell count= Cell density(cells/ml) x Volume of original cell suspension(ml) = Total cell count(cells) * This # could be 2, 4, or 8, it all depends on the number of square sections you count. This # represents of obtaining an average cell count between squares....
View Full Document

This note was uploaded on 04/10/2011 for the course BIOL 4202 taught by Professor Staff during the Spring '11 term at North Texas.

Ask a homework question - tutors are online