BMB 440 Exam II F010

BMB 440 Exam II F010 - 4 { BMB 440 Introductory Immunology...

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Unformatted text preview: 4 { BMB 440 Introductory Immunology EXAM2 1. Compare and contrast the characteristics of the anipn reanyiil dfi # I [6' W bmgiis’flcoCXWTdyonfiqm‘tg or whoa me 00 m Mmm‘k WW M Mmh (mu/milme mama be M i - ,- g S W, Wm mggsméw‘m :3: m8 ' ' .. ‘ \vxol “(0 W 10 New W M00 . , é \{i .\‘\ ((H h] (V, N. Hairy H. Otherth kn 6% (H_ ' 2, that is combinatorial \‘ 'j ‘ " ' joining and combinatorial association, what additional mechanisms contribute to the M diversity of immunoglobulins‘7 Which' CDRs are the major tar ets of these. (15 ' Mm seam Om «acumm «at ( ! f . h Wk, ____, V k \/ L/ *(L‘ H GAGA“ me‘QhMS (\ V pm 1 3‘>‘”§”i’\%’ WW“ W’Wtwufix .’ “m ‘U 'WWSwW (m 1% fi‘ 316%, Fmewy m mqmm/Mmm bwdltfa W \M fig cam W 5:0qu WW \IMmb ‘Vb V’A WWAQW WQS ‘VO’YQ Mung M MVDT III. Diagram a typical IgG molecule. Be sure to label all major structural features. What are the effects on this molecule of a brief exposure to the enzyme pepsin? (15 pts) «We £605 emu plaUn'bl hm lb mfér mm? 9m Em, (. a4 m) 13% mygfig 2%»? IV. Circle the we BEST answer or fill in the blank (2 pts each). C 1. Short gene segments found in between the VH and JH gene segments are referred to as the a. memory segments b. siecificit:l segegts d. self/non-self segment \7 2. Which o the following does not contribute to immunogenicity? . 'gh molecular weight . chemical complexity /c7forei ess . . e. -degra 1 - llity C 3. In adaptive Munity, haptens do not th Ki) 3) require carrier molecules in order to be immunogenic b) react with-s ecific antibodies when carriers are not employed. \r c) stimulate antibody res onses wrthout camers ( W YM, C / eact w1t speci c antibodies w en cames are employed. TD c) all of the above. W 0 none of the above 5“ \S‘bu b 4. Whic: (rte following would not bind to anfigen? _ \)( I'c) F(ab’)z . “k (300% d) Whole IgG A, 5. following has no V domains ) H chain c) F(ab’)2 d) Fab e) a and d F 6. Which of the following will bind to Fc receptors a) Fab 13) F(ab’)z c) Fe (1) Whole IgG ‘eandb 7. The immunoglobulin class produced first in an immune response is a) IgG b) IgA c) I E e; IgD C 8. The major antibody class found in mucosal secretion is IgA, but also found as minor component is a) IgG b: . IgD c) all of the above 9-10. e classes of antibody found on mature, but naive, B cells meg“ and Ar 11. Which cl 5 of immunoglobulin has subclasses'in both humans and mice? Eai b) IgA c) IgE r" d) IgM e) IgG and IgA E 12. An immunologic adjuvant is a substance that a) Reduces the toxicity of the immunogen. b) Enhances the immunogcnicity of the immuno’gen. c) Enhances immunologic cross-reactivity. d Enhance emato oiesis e) Enhances the immun , - - aainst the immunoen C 13. Which of the class(es) of immunoglobulin below has four constant domains and no hinge region? a) IgG [33.75% t 131 d) 1813 e) c and d C 14. Which class of immunoglobulin is least abundant in the sera? a) IgG b d) IgM 15. Papain digestion of an IgG preparation of antibody specific for the antigen hen egg albumin (HEA) will a) Lose its antigen specificity b) precipate with HEA c Lo c all interchain disulfi d) Produce two Fab ecules and one Fc fragment e one of the above W A 16. Wh' L .N s of immunoglobulin is most abundant in the sera? . ) IgA c) IgE (1) IgM 17. Which of the following would be expected to stimulate the greatest percentage of human T lymphocytes? 3) Bovine serum albumin (BSA) b) collagen “ l .. linA CONA w m._f acterla lipopolysacchan c e 'D 18. The following properties of human IgG are true except: a) It can be transported across the placenta b) It can participate in the activationof complement c It h thalf-li I classes d) It induces t e ormation of leukocytes 19. An Example of a superantigen is a) Bovine serum albumin (BSA) C 20. An example of a T cell mitogen is a) Toll-like Receptor 3 (TLR3) b) C-reactive rotein CRP . ' TS? \ - Staphylococcal enterotoxin A (SEA) e) Bacterial lipopolysaccharide (LPS) 6 21. An antibody made against the antigen tetanus toxoid (TT) reacts ‘with itleven when the TT is denatured by disrupting all disulfide bonds. A ‘second antibody against Tl“ fails to react when the TT is similarly denatured. The most likely explanation can be stated as follows: a Thefirstantibod u 0 ' ’ - . ' b) The first antibody is specific for the primary amino acid sequence of TT, whereas thesecond is secific for conformational de - u 'nants. ‘e second antibody is specific for disulfide bonds. d) The first antibody has a higher affinity for TT. F 22. A preparation of monoclonal antibodies all have the same a) idiotype . b) allotype i c) isotype d) antigen specificity e li t ' class . all of the above g none 0 e a ove D 23. The class-specific antigenic determinants (epitopes) of immunoglobulins are associated with a) L chains b) J chains ) ‘Disulfide bonds _ ( "in" 6 Variable regions E 24. The idiotype of an antibody molecule is determined by the amino acid sequence of the a) Constant region of the L chain b) Variable region of the L chain c) Constant region of the H chain tant re 'ons ofthe H and L chai ‘1!) variable regions of the H and L chains E 25. Injection into rabbits of a preparation of pooled human IgG could stimulate production of a) anti-y heavy chain antibody b) anti-K chain antibody c) anti-Fe antibody _._ . . 21 chain antibody \\N0)Q HZL 3p“ ulm‘ C N‘htvfl “Ii h) V) f h) Q (our. H71 D 26.The immunoglobulin class which is found as a pentarmn the circulation is ‘ f mgG } (amt/pillow laments on: I .1 mg" - ‘X M v” I W [{ ‘2pxfl um um“, wwmuc 13m ‘, Lu ’1 l “I l 3 (W ' (WWW J W?) Vac/10a: , fl ' ’ I I. ’ (It 1 ’1’ §\27. Which class of ungunoglobulin is mtg'gg‘ycgvgg r r J K , \“Qw‘nkJN‘iXu-Nl:rcchu \ / 5&7: M71 («EL mm 3 3\ s 0 C) {53% I “ Va; W ‘ by“ g [3 o 28. The role of polyethylene glycol (PE ) in hybridom monoclonal antibody production is to a) enhance antibody production ‘ a ' uccessful fusion products ‘ C) promote cell fusio - provr e essen - .u . . - bats for fusion products 29-30. How many chances does a de eloping B cell have to generate a ctional immunoglobulin heavy chain? é A functional light chain? 1‘ 'b ‘1 Q A \J) // fl / 95;; Wow / QT“ E- SW XE w u s _: éw‘fi: wires 5 9’ -*~ '~>— S? ...
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This note was uploaded on 04/11/2011 for the course BIOL 4201 taught by Professor Staff during the Fall '09 term at North Texas.

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BMB 440 Exam II F010 - 4 { BMB 440 Introductory Immunology...

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