BMB 440 Exam III F'09

BMB 440 Exam III F'09 - EXAM3 BMB 440 Introductory...

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Unformatted text preview: EXAM3 BMB 440 Introductory Immunology Fall 2009 1. Describe the sequence of events by which Class II MHC molecules acquire antigens for display. (10 pts) Class I MHC molecules rrcofln'izes «firing» Milan flint has been processed b He aéo'cfltic'mms. In «this graying, {lie {5an will I! is passed #mujnlle Vesicle which it. divialeo .‘m 3 ccmyarfuenic ingrmsinj deltagj’. M 10m} '1 whim is broken (imam info Qmsmenks Hui: an: 134% residues in law!» A6le “the A mulls an actual) Clip is 35H all,th +0 Hie Nelsen-bin '. cm / so 31a Hie hagmenls 85 not yammburelfl camp/u wlé/i MHC class}! 133076 #16:! m realign Dace {slit papilla Wmehég are reaJu in be cam [axed Add a]!!! fl W, 3% will disilm 44% 9119 wk“ Mp if HLA'D and fibula «We MHC molecule (Md remit; 1;! crcsenézi-liafl. I]. You cross two inbred strains of mice, a BALBIc (H—Z") mouse with a CBA (H-Z") mouse. What MHC (Class I and II) molecules will the F1 progeny of this mating express on its normal \ liver cells. BE SPECIFIC! (10 pts) m .flflL %M#K%/. / dd and My class I flak; Md Apkx class I . 9mm“ in 515 F. 5mm 1M m and kg anti cogniwdcy with Dir/gland /' Lété/ III. Describe the organization of the human MHC. Include MI-IC classes, regions, and gene groducts. (10 pts) ‘ ' ‘m hum“ MHCI consist 0‘? a. at ion {Ma 15 oh'v'udai 'm’co 5 domains with a fizvm'tcrosbbul'm magnum {int '15 commit? linked {o the at regiM- Thc at resians am about ‘30 amino ad 5 'm 393% and 15 $1M“ £2» a émnsmembmne segwni 4M norms-t 08 about 25' thrvf/wpic amino acids and a cflhplamic fail. The hmicroalohfin I FLQNM is similar, {:0 8w. at; 60min. The human MHC‘II is similar to {Jae HHCI) Mk the exceth {Mt He cha'm are at and 5 and, has at ska|k «Sim. MCI is a monomnc 3M)“; wish ‘1'» abut/Ruhr 'lmmunoqbbulinvrlkb domains. saw, on anti g regions an «be covalth linkcd\w'|% Z cgbylasmic fail Hut Ame; 4m to Jake hammemhanc segmenb L%/V,/4Léceg/4 ’/ MW %7 77/ X? What are the genetic contributors to diversity of TCR genes. How do these differ m contributors to diversity of Ig genes? (10 pts) V/ fluncgmnai Hexibliiiq J . 1/ 'ft- ration nuciwhd'e Addisw \J ./_V- “Stew nuslwhdc motilm’t ‘VI and J03 tetanbim " an 7/95: o'iJtir 1%; conérthZo/S a} {lie I cats 1‘: 4h fad fiat sand/if hgfex muffs-RM, 11467 ' '5 V % fig pad in ’ leUSIffl- V ~ W - I '/ .V'PW Ix) V. Circle the one best answer (2 pts each). 1. Which of the following tests is usually quantitative? a) western blotting b) double immunodiffusion @ single radial immunodiffusion d) immunoprecipitation 2. Which of the following is a precipitation in liquid? a) double immunodiffusion A b) single radial imirnmodiffusion © the interfacial (ring) test d) immunoelectrophoresis 3. Which of the following is most sensitive? Q)o assive agglutination (3.3 immunoclectrophoresis c) single radial immunodifi‘usion (1) direct agglutination 4. Which of the following cannot be used to measure antibody affinity? assive agglutination ' surface plasmon resonance c) equilibrium dialysis all can be used c) none can be used 5. A pepsin digest of anti-SRBC antibodies can agglutinate sheep red blood cells (SRBC) @Q, t 6. The point or region on a gel in which the concentration of antigen and antibody is ideal for precipitation to occur is the a) zone of antigen excess @ zone of equivalence c) zone of antibody excess d) end zone e) twilight zone t_/-\ Hi0 7. An assay for quantitating either antigen or antibody which makes use of enzyme-bound antibody and a substrate which gives a colored product is. a) RlA b) Flow cytometry c) SRID © ELISA e) PAMP 8. A type of precipitation in gel in which antigen and antibody diffuse towards each other from wells, establishing a concentration gradient and giving a visible line of precipitation where concentrations are ideal, is the a) single radial immunodifi‘usion (5) double immunodiffusion ‘6 ring test d) rocket immunoelectrophoresis e) immunoelectrophoresis 9. Indirect immunoflourescence microscopy is a more sensitive technique than direct immun scence. Tru ;' F e,- Choose fro the following to answer the next 9 questions about the complement system C3b JC3b-B-+ C3bBb+Ba C3—t C3a—C3b J C 1 q \f C3, factor B, and factor D J C4b2a3b ’C9 csa, 4a, and 5a Clr c1, C4, c2, and C3 JCZ—CZa-LCZb cs Cllnh J C3 MIRL C4BP v’ Factor I 10. The early components of the alternative pathway are WM" D » l 1. A reaction catalyzed by C1ql’zS: (XI); {:29 4 Q2 12 - 12. A reaction catalyzed by factor D is C3b} 9§LSLEh+Bg . 13. A C5 convertase is Mtg. g5 . 14. The early components of the classical pathway are (: Ii (‘u' r‘ 2; and C fi . 15. The terminal component of the membrane attack complex is {‘3 16. The component which binds to the Fc region of antibodies is t" in —lI_—' Mapain digestion of an IgG preparation of antibody specific for the antigen hen egg albumin (HEA) will 3) Lose its antigen specificity b) precipate with HEA c) Lose all interchain disulfide bonds @ Produce two Fab molecules and one PC fragment e) None of the above 16. Which class of immunoglobulin is least abundant in the sera? a) IgG 13) 13A © 12:5 d) IgM 17. Which of the following would be expected to stimulate the greatest percentage of human T lymphocytes? a) Bovine serum albumin (BSA) b collagen c Concanavalin A (CON A) Staphylococcal enterotoxin A (SEA) e Bacterial lipopolysaccharide (LPS) 18. The following properties of human IgG are true except: a) It can be transported across the placenta b) It can be cleaved by pepsin and yet remain divalent c) It has the longest half-life of all Ig classes @ It induces the formation of leukocytes c) It can participate in the activation of complement 19. An Example of a superantigen is a) Bovine serum albumin (BSA) C-reactive protein (CRP) Phytohemagglutinin (PHA) Staphylococcal enterotoxin A (SEA) e) Bacterial lipopolysaccharide (LPS) 20. An example of a T cell mitogen is Toll-like Receptor 3 (TLR3) b) C-reactive protein (CRP) @ Phytohemagglutinin (PI-IA) d) Staphylococcal enterotoxin A (SEA) e) Bacterial lipopolysaccharide (LPS) \ \ (p 21. An antibody made against the antigen tetanus toxoid (TT) reacts with it even when the TT is denatured by disrupting all disulfide bonds. A second antibody against "IT fails to react when the TT is similarly denatured. The most likely explanation can be stated as follows: a) The first antibody is specific for several epitopes expressed by 'I‘T. @ The first antibody is specific for the primary amino acid sequence of “PT, whereas the second is specific for conformational determinants. c) The second antibody is specific for disulfide bonds. d) The first antibody has a higher affinity for TI. 22. A preparation of monoclonal antibodies all have the same a) idiotype b) allotype c) isotype d) antigen specificity e light chain class (b all of the above g) none of the above 23. The class-specific antigenic determinants (epitopes) of immunoglobulins are associated with a) I. chains b) I chains c) ‘Disulfide bonds (1 H chains ’ariable regions 24. The idiotype of an antibody molecule is determined by the amino acid sequence of the a) Constant region of the L chain b) Variable region of the L chain c) Constant region of the H chain d) Constant regions of the H and L chain ©Variable regions of the H and L chains 25. Injection into rabbits of a preparation of pooled human IgG could stimulate production of a) anti-y heavy chain antibody b) anti-K chain antibody c) anti-Fe antibody d) anti-i. chain antibody © all are correct ...
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This note was uploaded on 04/11/2011 for the course BIOL 4201 taught by Professor Staff during the Fall '09 term at North Texas.

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BMB 440 Exam III F'09 - EXAM3 BMB 440 Introductory...

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