MolExam1_key05 - Spring 2005 Molecular Biology Exam#1...

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Molecular Exam 1, 2005 Page 1 of 7 Spring 2005 Molecular Biology Exam #1 - Learning the Tools http://www.bio.davidson.edu/courses/Molbio/Exams/2005/MolExam1_05.doc There is no time limit on this test, though I have tried to design one that you should be able to complete within 4 hours. You do not have to take this exam in one block of time; you may come and go as you wish. However, if you feel another course presents a conflict of interest, please discuss this with me. You are not allowed to use your notes, any books, any electronic sources except those specified in the exam, nor are you allowed to discuss the test with anyone until Wednesday Feb. 2, 2003. EXAMS ARE DUE AT 9:30 ON WEDNESDAY, FEBRUARY 2 in DANA 256 lab . You may use a calculator and/or ruler. The answers to the questions must be typed into this file unless the question specifically says to hand write/draw the answer in the space provided. If you do not place your answers in the appropriate locations, I may not find them. There are 4 pages for this exam, including this cover sheet. -3 Pts if you do not follow this direction: Please do not write or type your name on any page other than this cover page. Staple all your pages (INCLUDING THE TEST PAGES) together when finished with the exam. Name (please print here): ANSWER KEY Write out the full pledge and sign: "On my honor I have neither given nor received unauthorized information regarding this work, I have followed and will continue to observe all regulations regarding it, and I am unaware of any violation of the Honor Code by others." How long did this exam take you to complete (excluding typing)?
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Molecular Exam 1, 2005 Page 2 of 7 1 6 pts. 1. My grandmother has lost her molecular cloning manual and needs some help. She has been doing molecular biology most of her life, but with her age, her mind is not as sharp as it used to be. Please help her by providing the information requested below. a) How do you make a 400 mL of a 2X stock solution when the 1X solution is 0.15 M Tris; 200 mM NaCl; pH 8.0? 14.52 g Tris (0.3M) 9.36 g NaCl (0.4M) Dissolve in about 300 mL water, add HCl to pH of 8.0, add water to 400 mL final volume. b) Grandma needs to electrophorese some DNA from her transgenic tomatoes to see which one she should serve cousin Elmo who refuses to eat GMO’s. Remind her how to make a 200 mL gel that is: 0.35% agarose; 1μg/mLEtBr; 0.5X TBE.
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