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MolExam2_2010Key - Spring 2010 Molecular Biology Exam#2...

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Molecular Exam 2, 2010 Page 1 of 5 Spring 2010 Molecular Biology Exam #2 – Applying Lessons There is no time limit on this test, though I have tried to design one that you should be able to complete within 4 hours, except for typing. You are not allowed to use your notes, any books, any electronic sources except those specified in the exam, nor are you allowed to discuss the test with anyone until all exams are submitted on Monday March 22. EXAMS ARE DUE AT 12:30 ON MONDAY, MARCH 22 . You may use a calculator and/or ruler. The answers to the questions must be typed under each question unless the question specifically says to do otherwise. If you do not type your answers on the appropriate pages, I may not find them unless you have indicated where the answers are. I would like you to print your exam with your interwoven answers. Color ink is not required. -3 Pts if you do not follow this direction: Please do not write or type your name on any page other than this cover page. Staple all your pages (INCLUDING THE TEST PAGES) together when finished with the exam. Name (please print here): Anne Swerkey Write out the full pledge and sign: On my honor I have neither given nor received unauthorized information regarding this work, I have followed and will continue to observe all regulations regarding it, and I am unaware of any violation of the Honor Code by others. How long did this exam take you to complete (excluding typing)? Average was 84.5% Added 8 pts to everyone’s
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Molecular Exam 2, 2010 Page 2 of 5 8 pts. 1. Tell me how to make the following two solutions. You must show your work to receive partial credit for wrong answers. a. Make 25 mL of a 12% w/v EDTA, 2X TBE solution. Dissolve 3 g of EDTA in about 15 mL water. Then add 5 mL 10X TBE solution. Bring up to 25 mL with water. b. Your DNA solution is 1.2 μg/ μL in concentration. You need 100 μL of this DNA at a new concentration of 36 ng/μL. Tell me a good way to make this new DNA solution. Take 3 μL of the stock DNA solution and put it into 97 μL water. Done.
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