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Molexamanswers1_02 - Spring 2002 Molecular Biology Exam#1...

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Molecular Exam 1, 2002 page 1 Spring 2002 Molecular Biology Exam #1 - Learning the Tools There is no time limit on this test, though I have tried to design one that you should be able to complete within 4 hours, except for typing. You are not allowed to use your notes, any books, any electronic sources except those specified in the exam, nor are you allowed to discuss the test with anyone until Wednesday Feb. 6, 2002. EXAMS ARE DUE AT 11:30 ON WEDNESDAY, FEBRUARY 6 . You may use a calculator and/or ruler. The answers to the questions must be typed on a separate sheet of paper unless the question specifically says to write the answer in the space provided. If you do not write your answers on the appropriate pages, I may not find them unless you have indicated where the answers are. There are four pages for this exam, including this cover sheet. Please do not write or type your name on any page other than this cover page. Staple all your pages (INCLUDING THE TEST PAGES) together when finished with the exam. Name (please print here): Average = 86.5% Range = 100 – 40 Points Added = 7 Write out the full pledge and sign: How long did this exam take you to complete (excluding typing)?
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Molecular Exam 1, 2002 page 2 1 6 pts. 1. Imagine you are a senior looking for a lab job and you have 5 job applications on file at different labs. It is Friday afternoon and you return to your room to get ready for a small party with your best friends which begins at 9 pm. At 8:30, you notice there are some faxes on your desk. The five labs have asked you to perform these calculations and send back your response before midnight on Friday. Please answer these questions correctly so you can get 5 job offers and still go to the party with your friends by 9 pm. A table of formula weights is after the last question. a. Make 225 mL of a solution that is 2.5 M NaCl, 0.1 M Tris-HCl, pH 7.5, 100 mM EDTA. 32.9 g NaCl + 2.72 g Tris + 9.36 g EDTA; obtain about 170 mL water, add dry stuff, dissolve, pH with HCl to 7.5 and bring volume up to 225 mL b. Make 50 mL of a 0.5X TBE, 1 X EtBr , and 0.8% w/v agarose when you have 5 L of 10X TBE and 100 mL of a 1000X EtBr stock solutions.
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