MolExamkey_2_03 - Spring 2003 Molecular Biology Exam #2...

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Molecular Exam 2, 2003 Page 1 of 8 Spring 2003 Molecular Biology Exam #2 – Applying Lessons There is no time limit on this test, though I have tried to design one that you should be able to complete within 4 hours, except for typing. You are not allowed to use your notes, any books, any electronic sources except those specified in the exam, nor are you allowed to discuss the test with anyone until Monday March 24, 2003. EXAMS ARE DUE AT 11:30 ON MONDAY, MARCH 24 . You may use a calculator and/or ruler. The answers to the questions must be typed on a separate sheet of paper unless the question specifically says to write the answer in the space provided. If you do not write your answers on the appropriate pages, I may not find them unless you have indicated where the answers are. For the figures, I took photographs from journals, so you may detect warps or angles that seem odd. This is due to my need to reduce glare and shoot form about a 45 degree angle. Do not take this odd perspective into consideration for your answers. Ignore the tilted angles of the figures. When you are ready to take the exam, send me an email with the subject line of Molecular Test . This will generate an automated email telling you how to download the exam. -3 Pts if you do not follow this direction: Please do not write or type your name on any page other than this cover page. Staple all your pages (INCLUDING THE TEST PAGES) together when finished with the exam. Name (please print here): Write out the full pledge and sign: Here is the honor code "On my honor I have neither given nor received unauthorized information regarding this work, I have followed and will continue to observe all regulations regarding it, and I am unaware of any violation of the Honor Code by others." Average = 88% range from 100 – 70% How long did this exam take you to complete (excluding typing)?
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Page 2 of 8 6 pts. 1. Using the information below, tell me how to make: a. 300 mL solution of 1.5M NaCl, 50mM EDTA, 0.25M Tris pH 7.3. 26.3 g NaCl 6.24 g EDTA 9.07 g Tris Dissolve in about 200 mL water, pH to 7.3 with HCl, then bring final volume to 300 mL. b. 50mL of a 0.8% w/v agarose gel that is 0.75X TBE. 0.4 g agarose 3.76 mL 10X TBE 46.24 mL water microwave to melt, cool, and pour. FWs: NaCl = 58.5; EtBr = 394; EDTA = 416; Tris = 121; HCl = 36.5; agarose = 204. Other raw materials include SDS = stock solution of 20%; TBE = stock solution that is 10X; 10 pts. 2. Two antibodies were made against a fly protein that we’ll call Wow . The first antibody was called “Ab 11” and the second antibody was called “awd Ab”. The investigators used these two antibodies to probe western blots made from tissues of different species as labeled in the figure. Interpret these data as fully as you can. Your answer needed to contain the following information:
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This note was uploaded on 04/11/2011 for the course BIOL 3800 taught by Professor Gross during the Spring '03 term at North Texas.

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MolExamkey_2_03 - Spring 2003 Molecular Biology Exam #2...

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