Lec1_W11

Lec1_W11 - DNA replication DNA DNA polymerase Transcription...

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DNA DNA DNA polymerase mRNA Protein Transcription RNA polymerase Translation tRNA, ribosomes DNA replication
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You should know the following relative to prokaryotes –  see chapter 1 in From Genes to Genomes: Structure of DNA DNA replication: Primase, primer, DNA polymerase, 5’ to 3’ Transcription: Promoter(-10 and -35 sequences), RNA polymerase Translation: RBS (Shine-Delgarno sequence), ribosome
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In this class, we will be using heat  or NaOH to denature DNA.   The  temperature at which denaturation  occurs depends on the length and  the number of A and T versus C  and Gs DNA can re-nature once  temperature is lowered  or the  solution is neutralized if NaOH was  used
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DNA Replication in bacteria http://www.youtube.com/watch?v=teV62zrm2P0&NR=1
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Promoter region in prokaryotic genes Promoter is specific sequence in DNA that RNA  polymerase and associated factors recognize with high  affinity
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As mRNA is being transcribes, ribosomes can bind and  begin translation
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Lec1_W11 - DNA replication DNA DNA polymerase Transcription...

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