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ps8 - 10.What does it mean to say that the universal...

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PS 8, 1 Problem Set #8 BIMM100 Winter 2010 – Short Answers 1. If you are investigating a protein coding gene and need to create a library in order to isolate it, why would it be advantageous to create a cDNA library instead of a genomic library? 2. What features do expression vectors need that other vectors do not? 3. How can clones containing a gene of interest be identified in a genomic library? 4. Which of the techniques discussed in class for monitoring changes in expression rely on a labeled probe? 5. Which technique would you use to qualitatively observe differences in expression of many genes at the same time? 6. Where are pre-rRNA transcripts processed and ribosomes assembled? 7. Describe the important modifications that occur to form mature tRNA. 8. How many translation frames are possible in a specific mRNA molecule? 9. What is the polypeptide coded for by 5’ AUG GCU AAA AGG UAA 3’?
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Unformatted text preview: 10.What does it mean to say that the universal genetic code is degenerate? What is a synonymous codon? 11.If the anticodon of a tRNA is 5’ IAC 3’, what are all the possible mRNA codons that can be recognized? (make sure to indicate 5’ and 3’ ends of codons). Note: it may help to draw a picture. Which amino acid(s) do these codons code for? 12.If the mRNA codon is 5’ CAU 3’, what are all the possible anticodons that can be recognized? (make sure to indicate 5’ and 3’ ends). Note: it may help to draw a picture. 13.If a cell has fewer than 61 tRNAs, how are all the codons translated? 14.List the 3 stages of translation and the factors involved in each. 15.Name the three tRNA sites within a ribosome and indicate what process occurs at each site....
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