Lec46 - Lec46DNATechnologies BBiology 1222 io022 Haffie...

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Bio022 Biology 1222 Haffie 09/10 Lec 46 DNA Technologies
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Bio022 Biology 1222 Haffie 09/10 Transgenesis Moving DNA from one cell to another requires: A. Isolated DNA of interest (from some place) B. Vector (tool/ carrier to move the DNA) C. Mechanism of transfer to host cell (Getting DNA  and vector into the host cell) D. Selectable “marker” (someway to know your  successful)
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Bio022 Biology 1222 Haffie 09/10 DNA of Interest DNA to be transferred may be : A. Isolated (cut) directly from a genome B. created by reverse transcription of mRNA (called  “cDNA”) C. Synthesized in a lab (just make them)
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Bio022 Biology 1222 Haffie 09/10 Vectors DNA to be transferred is ligated into a “vector”  designed to  be accepted and replicated  in the  host cell. Vectors are modified or artificial plasmids viruses  or chromosomes. (F- plasmid can be modified to  turn into vectors) “Sticky ends” produced by digestion with  restriction endonucleases facilitate creation of  “recombinant DNA”  
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Bio022 Biology 1222 Haffie 09/10 Mechanisms of Transfer Moving DNA across host cell membrane/wall: A. Transformation of “naked” DNA (electroporation- DNA and cells are put in an electrical  field- DNA will move right through the cell)- cells can be forced or encouraged, to take  DNA from the environment. B.
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Lec46 - Lec46DNATechnologies BBiology 1222 io022 Haffie...

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