MCRO 251 Study guide for Quiz # 1 Tuesday lab_Spring 2011 Material from lab Powerpoint slides and the lab manual is fair game for the quiz! This study guide is to guide you in your preparation. Good luck! □ Know the different parts of the microscope o Know what is resolving power o Why do we use immersion oil? □ Know the difference between a light microscope and a phase contrast microscope o What can you observe with a phase contrast microscope? o Brownian vs. direction motion □ Know how to write the names of bacteria and be familiar with them. □ Know the gram staining procedure and why the staining works. o What color should gram positive and negative organisms be? Why? o What is a counter-stain? o Why is it important to counter-stain during a gram stain? □ Understand the principles/ purpose of negative stain, acid fast stain, spore stain, capsule stain □ What does aseptic technique mean? o
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