Microbiology 251-11 Labs 1-2

Microbiology 251-11 - M icrobiology 251 Laboratory Manual Spring 2011 Robert Twarog Denise Dalton Bruce Alexander Department of Microbiology and

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Microbiology 251 Laboratory Manual Spring 2011 Robert Twarog Denise Dalton Bruce Alexander
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Department of Microbiology and Immunology The University of North Carolina - Chapel Hill, NC MCRO 251 Laboratory Manual ii
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Preface Unlike most lab manuals published by textbook publishers, the Microbiology 251 Laboratory Manual describes the laboratory activities exactly as they are to be carried out here at UNC-CH. The various species of microorganisms and items of equipment described are those which will be used in the exercises. This should make it easier to focus on the principles demonstrated in each activity which makes the lab manual a more effective addition to the lecture. The experiments have been selected to introduce you to a variety of key laboratory techniques and principles concerned with bacterial isolation and growth, impact of viruses on their host cells, measuring immune response, etc. Once these basic techniques related to bacterial growth are mastered, they will be applied to examine the effects of antibiotics/chemicals on bacteria. Along the way, you will be given opportunities to apply these principles and procedures to the identification of unknown bacteria. Located on each of the benches in the lab room, A Photographic Atlas for the Microbiology Laboratory, by LeBoffe and Pierce (Morton Publishing) may be used as a supplement. It features numerous photomicrographs and extensive color plates of bacterial growth on various media. In addition, the underlying biochemical principles of the laboratory tests used in the Microbiology 251 labs are discussed in detail. This combination of a well-designed supplement with an in-house lab manual should be helpful. The laboratory exercises in this manual reflect the overview nature of the Microbiology 251 course. Though most of the labs relate to the techniques and principles of bacteriology, there are also exercises devoted to immunology and virology. Each section designated as a weekly lab session begins with the exercises to be completed and evaluated from the previous week. The discussion then moves into the new material for the week with a brief introduction for each exercise. Following the introduction, the specific materials used and procedures to be followed are described. In addition, tables to record data and questions to assess understanding are also included where appropriate. During the twelve lab sessions and the unknown identification process, you should acquire a basic understanding of how we handle and study medically important bacteria; using techniques fundamental to clinical microbiology as well as to molecular biology. It is our hope that you will both learn from and enjoy this lab experience and that you will emerge with a solid understanding of basic microbiology, as well as an appreciation of the critical role of microorganisms in our lives. General Laboratory Rules and Procedures:
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This note was uploaded on 04/12/2011 for the course MCRO 251 taught by Professor Lorrainecramer during the Spring '09 term at UNC.

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Microbiology 251-11 - M icrobiology 251 Laboratory Manual Spring 2011 Robert Twarog Denise Dalton Bruce Alexander Department of Microbiology and

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