3HH3 module 5 lecture 11-post

3HH3 module 5 lecture 11-post - Establishing polarity in...

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Slide 1 determining the oocyte: asymmetric cell divisions and localization of cellular determinants mRNA and protein transport into the oocyte Reorganization of the cytoskeleton mRNA localization Establishing polarity in the Drosophila egg:
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Slide 2 Oogenesis: formation of the egg
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Slide 3 http://www.molbio1.princeton.edu/schupbach/index.php?option=com_conte Red: actin Green: encore protein marks the oocyte Ovariole contains developing eggs at different stages:
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Slide 4 Single Drosophila ovariole anterior posterior Progressively mature egg chambers. Egg chambers contain 15 nurse cells, one oocyte (both germ line) and these are surrounded by an envelope of follicle cells (somatic).
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Slide 5 Huynh and St Johnston D, 2004. The Germarium oocyte
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Slide 6 Oocyte determination in Drosophila Formation of the 16 cell cyst
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Slide 7 ! Development of the germline cyst: 16 cell cyst: result of 4 mitotic divisions in which cytokinesis is incomplete; all are connected by cytoplasmic bridges Fusome: vesicle-rich organelle; unique to the germline cyst cells; grows during mitotic cyst divisions
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Slide 8 Fusome Fusomes are made of small membranous vesicles kept together by proteins such as α -spectrin, β -spectrin, and adducin-like protein. They span through the ring canals connecting the cells of the germline cyst, growing with each mitotic division. Fusomes arise from a spherical structure (organelle) called the spectrosome in the germline stem cell de Cuevas M, Spradling AC, Development 1998; 125:2781-2789
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Slide 9 The frst mitotic division is asymmetric: The cystoblast (the cell that will become the oocyte) inherits one third oF the Fusome From the germline stem cell (GSC). The segregation oF Fusome continues to be asymmetric resulting in the accumulation oF most Fusomal material in the oldest cell. This cell later becomes the “oocyte”.
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Slide 10
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Slide 11 Pro-oocyte (1) has more fusome than other cells and the fusome marks the anterior of the cell.
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This note was uploaded on 04/13/2011 for the course BIOL 3HH3 taught by Professor Mccelland&dej during the Winter '11 term at McMaster University.

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3HH3 module 5 lecture 11-post - Establishing polarity in...

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