3HH3 module 5 lecture 11-post

3HH3 module 5 lecture 11-post - Establishing polarity in...

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Slide 1 determining the oocyte: asymmetric cell divisions and localization of cellular determinants mRNA and protein transport into the oocyte Reorganization of the cytoskeleton mRNA localization Establishing polarity in the Drosophila egg:
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Slide 2 Oogenesis: formation of the egg
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Slide 3 http://www.molbio1.princeton.edu/schupbach/index.php? Red: actin Green: encore protein marks the oocyte Ovariole contains developing eggs at different stages:
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Slide 4 Single Drosophila ovariole anterior posterior Progressively mature egg chambers. Egg chambers contain 15 nurse cells, one oocyte (both germ line) and these  are surrounded by an envelope of follicle cells (somatic). 
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Slide 5 Huynh and St Johnston D, 2004. The Germarium oocyte
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Slide 6 Oocyte determination in Drosophila Formation of the 16 cell cyst
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Slide 7 Development of the germline cyst: 16 cell cyst: result of 4 mitotic divisions in which cytokinesis is incomplete; all are connected by cytoplasmic bridges Fusome: vesicle-rich organelle; unique to the germline cyst cells; grows during mitotic cyst divisions
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Slide 8 Fusome Fusomes are made of small membranous vesicles kept together by proteins such as α -spe ctrin, β -spe ctrin, a nd a dducin-like prote in. The y spa n through the ring ca na ls conne cting the ce lls of the ge rm line cyst, growing with e a ch m itotic division. Fusom e s a rise from a sphe rica l structure (orga ne lle ) ca lle d the spectrosome in the germline stem cell de Cuevas M, Spradling AC, Development 1998; 125:2781-2789
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9 The first mitotic division is asymmetric: The cystoblast (the cell that will become the oocyte) inherits one third of the fusome from the germline stem cell (GSC). The segregation of fusome continues to be asymmetric resulting in the accumulation of most fusomal material in the oldest cell. This cell later becomes
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3HH3 module 5 lecture 11-post - Establishing polarity in...

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