exp1 - INTRODUCTION: i. Objective: To determine phosphorous...

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INTRODUCTION: i. Objective: To determine phosphorous in its molecular form utilizing molecular spectrophotometer method. ii. Theory: The determination of Phosphorous uses the Molybdate as a complexing agent. The method is based on the spectrophotometer determination of phosphorous by reacting potassium dihydrogen orthophosphate with molybdate-vanadate in acidic media. A yellow colored complex will be formed absorbing at the wavelength of maximum absorbance ג max at 430 nm. This complex was found to obey Beers’ Low with absorbance proportional to the phosphorous concentration. To find the concentration of solution diluting by water by V1 x C1 = V2 x C2 (1) Procedure: The standard stock solution first was prepared Five working solution with reagent and different Concentrations were prepared The blank solution was prepared The absorbance of all solutions were measured Plot of absorbance vs. concentration is fitted The concentration of the unknown is determined
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exp1 - INTRODUCTION: i. Objective: To determine phosphorous...

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