Chemical Biology - Skeleton Notes

Chemical Biology - Skeleton Notes - CHEM 1AA3: Intro....

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Department of Department of 1 Chemistry Chemistry CHEM 1AA3: Intro. Chemistry II Chemical Biology Brainwashing Bees , Drug Discovery, and Aromaticity 2 Chem 1AA3 Queen Pheromone Blocks Aversive Learning in Young Worker Bees • Vergoz et al. Science ( 2007 ) 317:384-386 • queen mandibular pheromone (QMP) - causes young workers to feed and groom her - suppresses new queens, controls colony behaviour • read the accompanying file, "2010_Chembio_commentary.pdf"
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3 Chem 1AA3 The Biology of Brainwashing Bees • QMP contains homovanillyl alcohol (HVA) - HVA suppresses bad memories, but not good ones • Biological role: - mitigates unpleasant side effects of QMP (?) dopamine homovanillyl alcohol (HVA) 4 Chem 1AA3 The Biology of Brainwashing Bees • Significance • Therapeutic potential - high [dopamine] in some psychoses & schizophrenia - HVA-like molecules could be treatments BUT - need high specificity: – attention deficit disorder & Parkinson's disease are linked to low [dopamine] global suppression of [dopamine], or suppression in the wrong parts of the brain, could have undesired side effects
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5 Chem 1AA3 The Biology of Brainwashing Bees Key concepts • queen bees control worker bees' memories using HVA • help understanding human learning • develop new therapeutics 6 Chem 1AA3 The Chemical Biology of Brainwashing Bees • How do we go from a biological phenomenon to the development of a new therapeutic agent? • 1 new drug requires ~5000 failed compounds improving synthesis & accelerating assays facilitates drug discovery
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7 Chem 1AA3 High-throughput screening • high-throughput screening (HTS): – fast way to assay for a complex biological response • 2 components: • instead of doing experiments on live bees, use dopamine- producing bee neural cells growing in cell culture • a fast, colour-producing dopamine assay already exists 8 Chem 1AA3 High-throughput screening • 96 well microtiter plates allow 96 simultaneous assays • grow bee neural cells in wells of plates • test for dopamine production
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9 Chem 1AA3 High-throughput screening • some wells are used for standards and controls, others are for test compounds = high [dopamine] = no dopamine 10 10 Chem 1AA3 High-throughput screening • screening must be able to test thousands of compounds per day, therefore it must be automated using robots and microtiter plates McMaster HTS Facility
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This note was uploaded on 04/13/2011 for the course CHEM 1AA3 taught by Professor H during the Spring '08 term at McMaster University.

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Chemical Biology - Skeleton Notes - CHEM 1AA3: Intro....

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