micro report 4 - Enzyme-Linked Immunosorbent Assay Matthew...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Enzyme-Linked Immunosorbent Assay Matthew Ricke TA: Meg and Smita I ntroduction: The ELISA test is an Antibody based test. The purpose of this test is to show how an antibody will specifically attach to an antigen. I t is called enzyme-linked because the tail portion of antibody is where the enzyme is attached, this enyzmed- linked attachment catalyzes the colorless substrate into a colored product. To test for a certain infectious agent a sample of the fluid is loaded and the antigen is then added, a coloring agent is then added, this enzyme is responsible for a color change in a positive test result if no color appears then it is negative. Also, the intensity of the color shows how much reaction product occurred, or the concentration of the antigen in the well. The ELISA test is an important test that allows the detection of infectious diseases like that of H IV. (Barnett, 235) Procedure: In the first part of this lab there are two tubes with our body fluids in them, one for sharing and one labeled non-sharing. Next, we exchanged about 100 micro one for sharing and one labeled non-sharing....
View Full Document

This note was uploaded on 04/13/2011 for the course MICRO 400 taught by Professor Hierl during the Spring '11 term at Kansas State University.

Page1 / 3

micro report 4 - Enzyme-Linked Immunosorbent Assay Matthew...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online