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Unformatted text preview: Enzyme-Linked Immunosorbent Assay Matthew Ricke TA: Meg and Smita I ntroduction: The ELISA test is an Antibody based test. The purpose of this test is to show how an antibody will specifically attach to an antigen. I t is called enzyme-linked because the tail portion of antibody is where the enzyme is attached, this enyzmed- linked attachment catalyzes the colorless substrate into a colored product. To test for a certain infectious agent a sample of the fluid is loaded and the antigen is then added, a coloring agent is then added, this enzyme is responsible for a color change in a positive test result if no color appears then it is negative. Also, the intensity of the color shows how much reaction product occurred, or the concentration of the antigen in the well. The ELISA test is an important test that allows the detection of infectious diseases like that of H IV. (Barnett, 235) Procedure: In the first part of this lab there are two tubes with our body fluids in them, one for sharing and one labeled non-sharing. Next, we exchanged about 100 micro one for sharing and one labeled non-sharing....
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This note was uploaded on 04/13/2011 for the course MICRO 400 taught by Professor Hierl during the Spring '11 term at Kansas State University.
- Spring '11