WK5 QUESTIONS - 5 The amino-acyl tRNA synthetase enzymes...

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Biol204 Week5 Questions 1.) Be able to make a sketch and narrate in detail how replication proceeds on the leading strand using the following words (more or less in order of appearance): helicase, primase, RNA, DNA polymerase III, DNA polymerase I, DNA ligase. Be sure to explicitly state how hydrogen and covalent bonds are made and broken for each step. 2.) Be able to clearly sketch where and how Okazaki fragments are made. Use the words, polymerization, 3’ vs 5’ carbon, ligase, anti-parallel, Okazaki fragment, leading strand, lagging strand. 3.) Be able to make a sketch and narrate how transcription proceeds, using the following words (more or less in order of appearance): Promoter region, RNA polymerase, template strand, coding strand, GC hairpin. Be sure to explicitly state how hydrogen and covalent bonds are made and broken for each step. 4.) Given the genetic code, you should know how to translate any codon into an amino acid. You should also be able to list all tRNAs that specify a particular amino acid.
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Unformatted text preview: 5.) The amino-acyl tRNA synthetase enzymes match up specific amino acids with tRNA molecules, in preparation for translation of mRNA into protein. Please describe the specificity of this process. In particular, a. In principle, how many different tRNA molecules are there? b. How many different synthetases are used to prepare for translation? c. To how many different amino acids can a particular synthetase bind? d. To how many different tRNA molecules can a particular synthetase bind? 6.) Please narrate and make a sketch to describe how translation proceeds, using the following words (more or less in order of appearance): ribosome, mRNA, codon, anti-codon, tRNA, AUG, Methionine, P-site, A-site, peptide bond, translocate, E-site, stop codon, release factor. Be sure to explicitly state how hydrogen and covalent bonds are made and broken for each step. Hint, use Campbell’s Activity 17.4 to help you till you narrate the whole story without assistance....
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