Chapter15 - Biotechnology Chapter 15 Biotechnology The use of living creatures to help make products for humans Concept is very old but recombinant

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1 Chapter 15 Biotechnology Biotechnology The use of living creatures to help make products for humans Concept is very old, but recombinant DNA is new First modern use: Producing large amounts of human proteins such as hormones Tool: Restriction Enzymes Defensive proteins of bacteria Purified and used in labs Specific recognition sequences (often palindromes) GAATTC CTTAAG Sticky Ends Offset cuts on each side G AATTC CTTAA G Ends of DNA cut with same restriction enzyme are complementary (base pairing) Plasmids Small circular pieces of DNA that exist in bacteria Genes on plasmids expressed as usual (Some plasmids carry antibiotic resistance)
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2 Cloning Making exact genetic replicas The bacteria clone the human gene (and make the protein) Billions of copies overnight Reproductive cloning Entire organisms First mammal: “Dolly” the sheep Now: Goats, cats, dogs, etc. Steps of reproductive cloning 1. Remove nucleus from an egg 2. Fuse egg with somatic cell (diploid) 3. Implant fused cells in uterus 4. Baby grows as usual
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This note was uploaded on 04/15/2011 for the course BIOL 101l taught by Professor Huddleston,m during the Fall '08 term at University of Hawaii, Manoa.

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Chapter15 - Biotechnology Chapter 15 Biotechnology The use of living creatures to help make products for humans Concept is very old but recombinant

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