Microscopelab - Introduction to the Microscope Scientific...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
8 Introduction to the Microscope Scientific study depends upon direct observation of living things. But the human eye is limited, and can only distinguish or resolve objects of a certain size. For instance, if you can see that the dots in a colon (:) are separate, then you have the ability to resolve two objects that are about 1 millimeter or 1000 microns apart. With the invention of the microscope, scientists were able to resolve much smaller objects and see the cells and parts of cells that make up living things. The fields of science, medicine and technology have all advanced greatly due to knowledge gained by using microscopes. This exercise is intended to get you familiar with our microscopes and how to use them so you can see things you cannot see with your eyes alone. A. Parts of the light microscope Each student has an assigned microscope for his or her own use. The microscopes are numbered, and you should get the one that matches the number of the seat you are sitting in. (Be sure to return it to the proper numbered slot when you are done.) Carry it to your seat with both hands (you don’t want to pay for a broken microscope!). Plug the cord into the socket in front of your table and push the button on the microscope base to turn the light on (turn the light off when not in use). Locate the following parts of your microscope: The eyepiece , or ocular, is where you can look into the microscope. It holds a lens that magnifies objects ten times (10X). Some microscopes are monocular (one eyepiece) and some are binocular (two eyepieces). Does your microscope have one or two eyepieces? Between two and four objective lenses are mounted in a rotating nosepiece. Hold the ring above the objectives (not the objectives themselves) and rotate until you feel each objective click into place. If the objective is not properly in place, you will not see anything. Longer objectives have higher magnifying power. You may have a scanning objective, which magnifies 4 times (marked 4X). The low-power objective, which magnifies 10 times, is marked 10X. The high-power objective is marked 40X or 43X or 45X. You may have an oil-immersion objective, 100X, but we will not use it in this course. The total magnification is calculated by multiplying the eyepiece and objective magnification numbers. Examples: When you look through the eyepiece and the scanning objective, the total magnification is 40X. When you look through the eyepiece and the low-power objective, the total magnification is 100X. You can now answer the first worksheet question (see page 13) on total magnification. The stage is a platform that holds a slide for you to look at. Slides are 1 inch by 3 inch pieces of glass with the object to be viewed placed in the center and a smaller, thinner, piece of glass or plastic, the coverslip, placed over the object.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
9 Light from the illuminator passes through the condenser, the slide, the objective lens, and the eyepiece and then enters your eye.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/15/2011 for the course BIOL 101l taught by Professor Huddleston,m during the Fall '08 term at University of Hawaii, Manoa.

Page1 / 7

Microscopelab - Introduction to the Microscope Scientific...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online