Rev Q Exam II S'02 BT text

Rev Q Exam II S'02 BT text - Current Applications of...

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Current Applications of Biotechnology Biology 4770 Review Questions Exam II Spring 2002 1. What are some of the important considerations (features, properties, etc. of genes or vector which may be manipulated) in order to optimize the expression of cloned genes in prokaryotes? 2. Explain the importance of ________ in your choice of expression vectors (what is the function of each and why they are important/necessary). a. promoter (strength, regulation) b. ribosome binding sites c. transcriptional terminators d. origin of replication e. multiple cloning site f. selectable markers g. vector copy number 3. Briefly diagram a method for isolating a promoter sequence. 4. Give an example of two regulatable promoters used in expression vectors for prokaryotes and describe the advantages or disadvantages of each. 5. What is a shuttle vector? Why are these often necessary? 6. What is a fusion protein? Why are these often useful intermediates in the process of making certain proteins by recombinant DNA
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Rev Q Exam II S'02 BT text - Current Applications of...

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