Rev Q Exam III spr'05 BT text

Rev Q Exam III spr'05 BT text - Current Applications of...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Current Applications of Biotechnology Biology 4770 Review Questions - Exam III Spring 2005 Chapter 9 1. What is a monoclonal antibody and how are they made (explain and diagram the process). 2. Describe/explain/diagram an example of a DNA-based diagnostic system for detecting an infectious disease. 3. Describe/diagram how biotin can be used in a nonradioactive hybridization procedure to detect the presence of specific DNAs. 4. What is a molecular beacon and how do they work (diagram/explain and example)? 5. What is a RAPD and how are they now most often used in DNA profiling analyses (diagram/explain an example)? 6. Diagram/explain a protocol of how PCR can be utilized to screen for a genetic disease such as sickle cell anemia. 7. Describe/diagram how the mutation in the human beta-globin gene which gives rise to sickle cell anemia can be detected by RFLP? by PCR? by combining PCR and RFLP? 8. What is the ELISA technique and how does it work? Give an example of how it can be applied as a diagnostic procedure. 9. Give two examples of how the binding of DNA probes can be labeled/detected using a nonradioactive procedure. What advantages may this have over traditional radioactive detection procedures? 10. You have the assignment of developing a relatively simple, sensitive and reliable diagnostic test for early detection of a virus agent causing a dangerous epidemic. The virus has a duplex DNA chromosome and is found at an early stage in the blood stream of infected individuals. Your test must be able to detect very low levels of virus present prior to the onset of symptoms in order to reduce spread and allow early treatment. Describe/diagram the protocol and explain why you have chosen this approach. 11. Describe an example of how bacteria might be used as biosensors. 12. Describe/diagram the first DNA-based test for carriers (or homozygotes) for a human genetic disease.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
13. Describe/diagram how the mutation in the human beta-globin gene which gives rise to sickle cell anemia can be detected by RFLP? by PCR? by combining PCR and RFLP? 14. Describe/diagram and explain the RFLP testing procedure as applied commonly to human identity testing. Provide a possible example of test results. 15. Describe/diagram a PCR based test commonly used for identity testing. Provide sample results. 16. Briefly describe 3 areas where DNA profiling will be expected to (or already does) have major applications. Chapter 10
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 6

Rev Q Exam III spr'05 BT text - Current Applications of...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online