Exam II, Spr '02 MB text

Exam II, Spr '02 MB text - recognized by the protein 2(25...

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BIOL/BIOC 4570/5340 Biochem. and Mol. Biol. of the Gene Spring 2002 Exam II Exam Scoring Record your answers in the spaces provided. If you require more space for any of the essay 1. _______/10 questions, use the back of the page on which the question is printed. Read each question 2. _______/25 carefully. The essay questions often have multiple parts. Make sure you have answered 3. _______/25 each of them so that you will not lose points due to simple omission. The maximum points 4. _______/25 possible on this exam is 110 (including 10 bonus points) 5. _______/25 _________/110 Total Points 1. Short Answer Questions (2 pts each) a. CAP protein in E. coli is activated by: _____________________________. b. The RNA polymerase core enzyme consists of: ___________________________. c. The DNA sequence at the -10 region of an E. coli vegetative ("standard") promoter: ___________________________. d. The small signal molecule produced by E. coli cells as part of the stringent response is: __________________________. e. The E. coli origin of DNA replication is specifically
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Unformatted text preview: recognized by the protein: ____________________________. 2. (25 pts) Describe/diagram the regulation of the trp operon of E. ` coli. 3. (25 points) Describe and diagram the process which the E. coli bacteriophage fX174 produces new single-stranded chromosomes from the original chromosome which enters the bacterial cell. 4. (25 pts) An E. coli cell is infected by a lambda bacteriophage. Describe (and diagram as necessary)the series of molecular events , which leads to the formation of a lysogenic relationship with the bacterial cell. 5a. (10 pts) How can antisense RNAs be utilized to experimentally regulate gene expression in eukaryotic cells? Why is this often more useful than simply creating a mutant defective in this gene product by traditional means (as generally used for bacterial systems)? 5b. (15 pts) Give (explain/diagram) of where antisense RNAs are used naturally by an organism to regulate gene expression...
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Exam II, Spr '02 MB text - recognized by the protein 2(25...

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