Selective Media Assignment

Selective Media Assignment - Selective Media Assignment...

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Unformatted text preview: Selective Media Assignment During this week's lab, students will be investigating the use of selective media, which are microbiological media designed to grow only specific types of bacteria. The growth selectivity is generally between Gram positive and negative, but some allow for even more selectivity. Students are encouraged to make Gram stains of the organisms present and record the results of these Gram stains in their lab notebook for future reference. Students may work in groups to complete this task, each student taking a different set of organisms to Gram stain. As always, replicates are encouraged. Procedure: Selective Media Students will work in teams of 4 to inoculate, observe and document the selective media. You will use the media & organism combinations listed below. All of the given organisms will be inoculated onto a single plate. NOTE: The following represents common organisms used as controls for these tests. Due to culture availability, there may be different organisms present during lab. Make a note in your lab notebook of the organisms you actually use on the plates. 1. Mannitol Salt Agar (1 per 2 students) o Staphylococcus aureus o Staphylococcus epidermidis o Escherichia coli 2. Phenylethyl Alcohol Agar (1 per 2 students) o Staphylococcus aureus o Escherichia coli o Enterococcus faecalis 3. Eosin Methylene Blue Agar (1 per 2 students) o Escherichia coli o Enterococcus faecalis o Salmonella typhimurium 4. Hektoen Enteric Agar (1 per 2 students) o Escherichia coli o Enterococcus faecalis o Salmonella typhimurium o Shigella sp. 5. MacConkey Agar (1 per 2 students) o Escherichia coli o Enterococcus faecalis o Proteus sp. o Shigella sp. 6. Desoxycholate Agar (1 per 2 students) o Escherichia coli o Enterococcus faecalis o Salmonella typhimurium o Shigella sp. ...
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