BCH1048 Tutorial 2 - Gene cloning Gene ( ) Gene donor...

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Gene donor Plasmid ( 质粒 ) donor Isolation of bacterial plasmid Plasmid opened with restriction enzyme ( 限制酶 ) Isolated gene DNA ligase ( 连接酶 ) binds ends together Recombinant ( 重组 ) DNA molecule Chromosome ( 染色体 ) Transformation ( 转殖 ) of fresh bacterium Donor gene Transcription mRNA Translation Protein product Gene cloning Gene cloning ( 基因克隆 基因克隆 )
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Generation of protein Generation of protein -coding gene region for cloning coding gene region for cloning Cell Test tube Exon 编码顺序 Intron 基因内区 Splicing Reverse transcriptase 逆转录酶 : mRNA cDNA
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A typical animal cell A typical animal cell Cytoskeleton ( 细胞骨架 ) Not in most plant cells
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A typical plant cell A typical plant cell Not in animal cells Cytoskeleton ( 细胞骨架 )
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A typical bacterial cell A typical bacterial cell
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Nucleus ( 细胞核 ) Ribosome ( 核糖体 ) Golgi apparatus ( 高尔基体 ) Plasma membrane ( 细胞膜 ) Mitochondrion ( 线粒体 ) Smooth endoplasmic reticulum ( 滑面内质网
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This note was uploaded on 04/16/2011 for the course BCH 1048 taught by Professor Prof.cheng during the Fall '08 term at City University of Hong Kong.

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BCH1048 Tutorial 2 - Gene cloning Gene ( ) Gene donor...

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