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BCH1048 Tutorial 2 - Gene cloning Gene Gene donor Plasmid...

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Gene donor Plasmid ( 质粒 ) donor Isolation of bacterial plasmid Plasmid opened with restriction enzyme ( 限制酶 ) Isolated gene DNA ligase ( 连接酶 ) binds ends together Recombinant ( 重组 ) DNA molecule Chromosome ( 染色体 ) Transformation ( 转殖 ) of fresh bacterium Donor gene Transcription mRNA Translation Protein product Gene cloning Gene cloning ( 基因克隆 基因克隆 )
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