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1MolecularRev.m11 - b RNA levels i RNA gel(northern Blot ii...

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PLB 113D Discussion Section April 4-7, 2011 Discussion Section #1 Molecular and Genetic Approaches to Study Plant Biology The TAs will lead a discussion of molecular and genetic approaches to study plant biology. The following topics will be covered. 1. Basic Steps in Gene Expression a. Transcription b. mRNA production c. Translation 2. Nucleic Acid Cloning a. cDNA (mRNA sequences) b. Genomic DNA 3. Nucleic Acid Hybridization 4. DNA Analysis a. DNA gel (Southern) blot hybridization b. Polymerase Chain Reaction 5. Measuring Gene Expression a. Promoter activity i. Chimeric genes
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Unformatted text preview: b. RNA levels i. RNA gel (northern) Blot ii. RT-PCR c. Protein activity 6. Genetics a. Genetic loci and alleles b. Recessive and dominant mutations 7. You can check the following websites to get information about molecular techniques. a. Dolan DNA Learning Center (Gel electrophoresis, Cloning, PCR, lots of stuff!) http://www.dnalc.org/ddnalc/resources/animations.html b. Raven Biology Text Website (Chapter 16 links) http://highered.mcgraw-hill.com/sites/dl/free/0072437316/120060/ravenanimation.html...
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