C5 Quality assurance and Calibration Methods

C5 Quality assurance and Calibration Methods - 05 Quality...

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05 Quality assurance and Calibration Methods Dr. Han 1 Dr. Han
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5.1 Calibration Methods (1). Calibration Curves : A graph of the response versus concentration of analyte. 2 Dr. Han Method of Least Squares:
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5.1 Calibration Methods (1). Calibration Curves : A graph of the response versus concentration of analyte. Dr. Han 4-G In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to protein. Absorbance of light is measured. Protein, μ g A 595 0.00 0.466 9.36 0.676 18.72 0.883 28.08 1.086 37.44 1.280 y = 0.0218x + 0.4706 R² = 0.9998 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3 1.4 0 10 20 30 40 Absorbance at 595 nm Protein, m g Calibration Curve of Protein Determination ch4 Calibration curve.xlsx
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4 Dr. Han
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5.1 Calibration Methods (2). Standard Addition 5 Dr. Han C ClO 4 - >=18 μg /L in drinking water is of concern because it can reduce thyroid hormone production! Matrix effect: A change in analytical signal caused by anything in the sample other than analyte.
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6 Dr. Han Standard Addition : A technique in which an analytical signal due to an unknown is first measured. Then a known quantity of analyte is added, and the increase in signal is recorded. From the response, it is possible to calculate what quantity of analyte was in the unknown.
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7 Dr. Han
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Dr. Han 8
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Dr. Han 9 When y= 0, x=[S] i ( V / V 0 = −[X] i
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5.1 Calibration Methods (3). Internal Standard 10 Dr. Han An
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This note was uploaded on 04/18/2011 for the course CHEMISTRY 210 taught by Professor Dr.han during the Spring '11 term at Fayetteville State University.

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C5 Quality assurance and Calibration Methods - 05 Quality...

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