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Lecture_4-_Electrical_activity_in_neurons

Lecture_4-_Electrical_activity_in_neurons - Ki-67 is a...

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Unformatted text preview: Ki-67 is a protein found in actively proliferating cells, from G1, Sand G2phases, through mitosis.It is not found, however, in G, which is typical of terminallydifferentiated cells that are not dividing.Clarification on Ki-67Growth and synthesisGif cell terminallydifferentiatesandwon’t divide further.Deriving Neurons From ESC’shESC hESC-NPC hESC-neuronPurification of human neurons from human ESCsLight microscopy (phase contrast) of ESC’s differentiating.Differentiating ESC’s entails changing the environmental signalshESC hESC-NPC hESC-neuronPurification of human neurons from human ESCsUsing immunofluorescence we can quantify the efficiency of differentiation.Sox2NPC markerKi67proliferation markerTuj1neural markerMAP2neural markerTesting the Functionality of NeuronsHuman ESC-derived neurons are electrically activeSpiking action potentials when depolarizedA fully differentiated and functional neuron will have electricalactivity:Measuring action potentials.Human ESC-derived neurons are electrically active...
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Lecture_4-_Electrical_activity_in_neurons - Ki-67 is a...

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