Review for Lab Final Exam

Review for Lab Final Exam - EPIDEMIOLOGY SIMULATION AND...

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EPIDEMIOLOGY SIMULATION AND ELISA Exercise 41: Epidemiology 1. Epidemic: outbreak of a disease that occurs within a relatively short period of time at an unusually high frequency in a given population. 2. Epidemiology: study of epidemics 3. Index case: first recorded incidence of a disease within a given population 4. Objective: isolate infected individuals in order to prevent further transmission through the population 5. Example: Lyme disease, successful epidemiological breakthrough 6. Although the transfer of antibodies does not cause disease, your immune system typically produces antibodies following exposure to a disease agent. Procedure: 1. Exchange of fluid: a. Vortex between each exchange b. Pasteur pipet 10 drops for each exchange c. Record # of all exchanges 4 times ELISA 1. Employs the use of secondary antibodies that are conjugated to a specific detector enzyme 2. Used to determine if an individual has been exposed to a particular pathogen whose presence triggered production of the antibodies 3. Example: HIV testing ELISA Process: to determine if Ab-Ag complexes will form 1. Wash initial wells with buffer 3 times, Fill with your number and a control 2. Incubate for 15 min at 37 degrees a. Maximizes the absorption of reactive antibodies to plastic wells 3. Wash all wells 3 times a. Removes any excess antigen that is unbound to well surface
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4. Fill all wells with secondary (conjugated) antibody 5. Incubate at room temp for 10-15 min 6. Wash with buffer 3 times 7. Add substrate to well and wait 2-5 min a. Positive: Green means antibody was present b. Negative: No reaction, stays clear Determining Index Case: 1. List all positive results including their number and who they traded with a. Go through positive result #’s and if any positives traded with a negative #, they cannot be the index b. For the final couple make flow charts to determine their order of transfer to eliminate until you find the index
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LAB SAFETY, WASTE DISPOSAL, AND GENERAL TECHNIQUES 1. Red Biohazard container (used items): a. microscope slides b. toothpicks c. Pipets without wrapper d. Microcentrifuge tubes e. plastic disposable screw/snap cap tubes f. oxidase test cards g. swabs w/o wrapper h. syringes w/o needle i. contaminated broken glassware j. any other used plastic, disposable material 2. Large Biohazard Container on Cart a. Petri dishes w/ microbial cultures b. Used plastic screw/snap cap tubes c. Contaminated paper towels used to clean up spilled cultures d. Used gloves e. Stomacher bags containing diluted food samples 3. Disposal Cart
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a. Tubes containing used microbial cultures i. Sorted by size, tape removed, cap reusable b. All used glassware 4. Broken Glassware Container a. Uncontaminated broken glassware 5. Regular Trash Bins a. Used non-recyclable paper b. Uncontaminated paper towels c. Gauze d. Extinguished matches e. Wrappers All tubes labeled with white label tape Do not label tubes on cap
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This note was uploaded on 04/19/2011 for the course MICROBIOLO 509 taught by Professor Madhuramilindpradhan during the Spring '11 term at Ohio State.

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Review for Lab Final Exam - EPIDEMIOLOGY SIMULATION AND...

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