DNA Binding and Dissociation

DNA Binding and Dissociation - 1 BME 100L: Topic 3 DNA...

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Unformatted text preview: 1 BME 100L: Topic 3 DNA Binding and Dissociation Watson Crick Base Pairing 3 H-bonds (stronger) 2 H-bonds (weaker) 2 Polymerase Chain Reaction (PCR) y Genome y Genome Sequencing y Molecular Cloning y Gene Therapy y Diagnosis of Infection & Genetic Disease Polymerase Chain Reaction (PCR) 3 DNA Association Problem: How to determine energy changes for DNA Problem: How to determine energy changes for DNA association/dissociation Application: Knowning optimal properties for designing DNA sequences for PCR, etc. How to calculate G, H, S for DNA association Experimental results show that primary sequence does not determine the thermodynamics DNA Association 5 ATAGCA 3 5-ATAGCA-3 3-TATCGT-5 + 5-ATAGCA-3 3-TATCGT-5 Nearly infinite number of sequence possibilities Can we determine a general method to calculate thermodynamic properties for all possibilities? 4 DNA Association Figure 3.6 Figure 3.7 DNA Association 5-ATAGCA-3 5 ATAGCA 3 3-TATCGT-5 + 5-ATAGCA-3 3-TATCGT-5 + =...
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This note was uploaded on 04/21/2011 for the course BME 100 taught by Professor Yuan during the Spring '07 term at Duke.

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DNA Binding and Dissociation - 1 BME 100L: Topic 3 DNA...

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