Crithidia bombi - 161 M-A03 129 129 127 127 122 138 135 137...

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Click to edit Master subtitle style 4/22/11 A Quantitative In Vitro Cultivation Technique to Determine Cell Number and Growth Rates in Strains of Crithidia bombi , a Parasite of Bumblebees Mario Popp H. Michael G. Lattorff
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Click to edit Master subtitle style 4/22/11 Crithidia bombi 10um
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4/22/11
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4/22/11 Scientific classification Domain: Eukaryota (unranked): Excavata Phylum: Euglenozoa Class: Kinetoplastea Order: Trypanosomatida Genus: Crithidia
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4/22/11 10-1 plate 1: 3 colonies 10-2 plate 1: 1 colony 10-1 plate 2: 5 colonies 10-2 plate 2: no colonies 10-1 plate 3: 5 colonies 10-2 plate 3: 1 colony All in all, there were 15 samples for genotyping, from which 6 turned out to be different genotypes.
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4/22/11 Cells*ml-1 Fig. 1. Correlation of optical density with cell
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4/22/11 Table S1. List of genotypes Cri 1.B6 Cri 2.F10 Cri 4 Cri 4G9 Size1 Size2 Size1 Size2 Size1 Size2 Size1 Size2 M-A01 125 131 125 125 122 140 151
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Unformatted text preview: 161 M-A03 129 129 127 127 122 138 135 137 M-A06 129 129 n.d. n.d. 130 136 139 157 M-A11 129 131 n.d. n.d. 118 138 155 157 M-A12 129 151 113 117 130 138 141 163 M-B01 131 131 127 127 134 136 147 149 4/22/11 Table S2 strain M-A12 (dilution 10-3) and strain M-A06 (dilution 10-2) with their absorbance at 600 nm (OD) and their corresponding cells per ml. Starting point for the six hour interval was set at 0h. 0h 1h 2h 3h 4h 5h 6h M-A12 absorbance 0.0130 0.0183 0.0540 0.0980 0.1455 0.1590 0.1593 cells*ml-1 27208 41208 135510 251737 377209 412869 413662 M-A06 absorbance 0.0143 0.0175 0.0265 0.0385 0.0560 0.0707 0.0730 cells*ml-1 30642 39094 62868 94566 140793 179623 185699 4/22/11 Ce • Fig. 2. Comparison of the growth rate of the two strains of Crithidia • bombi. Cells were counted 4/22/11 Questions???...
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