Unformatted text preview: molecules mainly according to their mass , because “shape” and charge differences have been negated. Sodium dodecyl sulfate (SDS): detergent; unfolds or “denatures” the protein • Denature: process in which proteins or nucleic acids lose their tertiary structure and secondary structure by application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), or heat PAGE: polyacrylamide gel elecrophoresis o Two-dimensional electrophoresis: discriminates charge and mass; do isoelectric focusing in a small cylindrical gel, place that gel on top of a SDS-PAGE gel, and do electrophoresis o Dalton (Da): 1/12th the mass of a carbon 12 atom; kiloDalton (kDa) is used as the standard measure of molecular mass for proteins...
View Full Document
- Spring '11
- molecular biology, polyacrylamide gel elecrophoresis