This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: wash proteins through column (small proteins attach to column), collect fractions (large proteins) o Ion exchange chromatography: preparation by charge; layer sample on column, negatively charged proteins attach to positively charged column, collect positively charged proteins, elute with NaCl, collect negatively charged proteins + Na+ o Affinity chromatography: preparation by specific binding; layer sample on column, add antibodies + pH 7 buffer, wash (collect protein which is not recognized by antibody), treat with pH 3 buffer, collect antibody-recognized protein Lateral flow immunochromatography: used in anthrax/pregnancy tests X-Ray crystallography: c rystalize your protein, blast it with a high-energy beam of x-rays, regular arrangement of electrons in the crystal diffracts the beam; use the diffraction pattern to determine an electron density map, thread the primary sequence through the electron density map...
View Full Document
- Spring '11