Enzyme Kinetics - A ndrew Castleman Enzyme Kinetics 2/14/11...

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Andrew Castleman 2/14/11 Enzyme Kinetics Acid phosphatase is an enzyme that catalyzes the hydrolysis of a single phosphate group. When mixed with para-nitrophenolphosphate, it will produce para-nitrophenol. If base is then added, it will convert the para-nitrophenol to para-nitrophenolate which has a yellow color to it. This color can be used to examine the amount of para-nitrophenol produced by the enzyme during a given amount of time with a known concentration of product. This reaction requires a cofactor, MgCl 2 , and works best a a pH below 7. The enzyme will then stop if the solution is made extremely basic. There are two major types of inhibitor, competitive and non-competitive. When added into the reaction, there should be a noticeable, predictable change in the rate of the enzyme. Competitive inhibitors act by blocking the active site of the enzyme so that the substrate cannot bind. This is overcome by an increase in substrate concentration. Non- competitive inhibitors bind at an allosteric site that causes the enzyme to change conformation so as not to allow the substrate to bind. This type of inhibition is not overcome
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This note was uploaded on 04/22/2011 for the course BIOCHEM 412 taught by Professor Thomas during the Spring '11 term at Tennessee Martin.

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Enzyme Kinetics - A ndrew Castleman Enzyme Kinetics 2/14/11...

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