Ouchterlony - Observe which wells have a white line...

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Andrew Castleman Ouchterlony The Ouchterlony test allows antibodies to form a network as they react with antigen to form a precipitate. As the antibodies and antigen diffuse towards each other, they interact and if the antibody responds to the antigen it should form the precipitate. Here this will be used to determine the closeness of the relation between cows and certain other animals. On an agar plate, make a well in the center of the plate and in a circle around the center make more wells for each sample. Fill the center well with antibody that was produced in a reaction to cow antigen. In each of the wells surrounding the center, fill them with the antigens of the animals of interest. Place the well in a cool place and wait for the diffusion process to occur.
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Unformatted text preview: Observe which wells have a white line perpendicular to an imaginary line between them and the center well. Sample Reacted Goat Yes Chicken No Donkey No Horse No Cow Yes Sheep Yes In our experiment, we used the following antigens: Cow, Chicken, Horse, Sheep, Goat, and Donkey. Only the Goat, Cow, and Sheep antigen reacted with the Cow antibody. This suggests that the epitopes on the Goat and Sheep serum are similar enough to Cow that the antibody is able to recognize the antigen and form the precipitate. The similar epitopes are highly indicative of a close evolutionary relationship. The results are as expected from other data indicating evolutionary relationships....
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This note was uploaded on 04/22/2011 for the course BIOCHEM 412 taught by Professor Thomas during the Spring '11 term at Tennessee Martin.

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