{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

SDS PAGE - A ndrew Castleman SDS PAGE Sodium dodecyl...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Andrew Castleman SDS PAGE Sodium dodecyl sulfate is a detergent that is able to unravel proteins by disrupting all non-covalent bonds and interactions the protein has with itself. When the amphiphilic SDS interacts with the protein it gives the overall complex a very large negative charge to molecular weight ratio. The charge to mass ratio is constant for all polypeptides. So much SDS interacts with the protein that the charge of the complex is essentially due to how many SDS molecules are in the complex. The negatively charged, denatured protein can then be studied using electrophoresis in a polyacrylamide gel. The proteins will separate by size as the smaller proteins will move more easily and the larger proteins less easily resulting in the smaller proteins traveling farther. This allows us to determine the number of distinct subunits formed when SDS denatures a purified protein and when compared to a standard containing proteins of known molecular weight, provides a way to estimate the molecular weight of the subunits/protein.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern