ch15_lecture - Recombinant DNA and Genetic Engineering...

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Recombinant DNA and Genetic Engineering Chapter 15
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Lipoproteins * Form when certain blood proteins combine with cholesterol High-density lipoproteins (HDLs) Low-density lipoproteins (LDLs)
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Genetic Changes* Humans have been changing the genetics of other species for thousands of years Artificial selection of plants and animals Natural processes also at work Mutation, crossing over
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Genetic Engineering * Genes are isolated, modified, and inserted into an organism Made possible by recombinant technology Cut DNA up and recombine pieces Amplify modified pieces
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Specificity of Cuts * Restriction enzymes cut DNA at a specific sequence Number of cuts made in DNA will depend on number of times the “target” sequence occurs
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Making Recombinant DNA * 5’ 3’ G C T T A A A A T T C G G A A T T C C T T A A G 3’ 5’ one DNA fragment another DNA fragment 3’ 5’
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Making Recombinant DNA* nick 5’ 3’ 3’ 5’ G A A T T C C T T A A G nick G A A T T C C T T A A G DNA ligase action
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ch15_lecture - Recombinant DNA and Genetic Engineering...

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