BactTranspring 2011

BactTranspring 2011 - BSC 2010L - BACTERIAL TRANSFORMATION...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BSC 2010L - BACTERIAL TRANSFORMATION TRANSFORMATION Plasmids Plasmids • Bacteria have extra genetic material Bacteria plasmids. plasmids – Plasmids are replicated during cell division. – The genes found on plasmids are The expressed, giving that bacterium additional characteristics. Plasmids typically have only 1, 2 or 3 genes. only Plasmids Plasmids Restriction enzymes cleave DNA Restriction Bacterial Transformation Today’s Lab 1. Digest plasmids 2. Bacterial transformation Bacterial Transformation lab exercises exercises We do 2 basic exercises in this lab: We 1. A restriction enzyme digestion of a bacterial plasmid. • Compare 3 digested (B,E &H) and 1 undigested plasmids with gel electrophoresis 2. Bacterial transformation: introduce plasmids with antibiotic 2. ntroduce resistance to E. coli. E. • Plate bacterial cells onto agar with ampicillin to screen for Plate transformed cells Where do we digest the plasmid? 2. Bacterial Transformation 2. • E. coli - not resistant to antibiotics. • Bacterial cells are competent, i.e., they are ready to Bacterial take up extraneous plasmids. take • Plasmids - with a gene giving bacteria resistance to ampicillin. ampicillin • Once plasmids are internalized, their genes are Once expressed by the bacterium - antibiotic resistance = transformation. transformation Write an essay about this lab (5 points) Write • The purpose of these two experiments • Methods including the strategy of a control Methods in both experiments in • What are your predictions and results • One page, single spaced, Ariel 12pt One Complete the Drosophila prelab handout before Drosophila the next lab. the ...
View Full Document

This note was uploaded on 04/24/2011 for the course BSC 2010L taught by Professor Herrerabaerbolker during the Spring '08 term at University of Florida.

Ask a homework question - tutors are online