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Unformatted text preview: BSC 2010L CELLS AND PROTISTS CELLS In this lab we will: In
A. Review compound microscope Review (Chapter 1). (Chapter B. Review cell structure and function Review (Chapter 2). (Chapter D. Begin examining diversity of life with D. some protist taxa (Chapter 7). some Microscopy Microscopy
A. Compound - binocular A. Compound B. Stereoscope - binocular dissecting scope B. Stereoscope Dissecting scopes Dissecting
A. Putting prepared slides under the A. dissecting scope is often a convenient way to look at larger specimens. way Principal Biological Concepts to Emphasize: to
A. Cell theory - all living things are made of Cell cells. cells. B. Cell types B. Cell C. Cell structures and functions C. Cell D. Diversity of some protist groups D. Diversity Cell Structure Cell
A. Basic aspects of cell structure and A. function function 1. All cells have a nucleus (or All nucleoid), cytoplasm and a plasma membrane. membrane. The plasma membrane isolates the cell from the environment, controls the flow of molecules into and out of the cell, and contains receptors that effect cell’s activities. activities. A nucleus is bound be a membrane and contains DNA. (In bacterial and blue-green cells, DNA in located in the nucleoid region and is not membrane-bound.) membrane-bound.) The cytoplasm contains membrane systems, particles, filaments (the cytoskeleton), and a semifluid matrix. and A prokaryotic cell prokaryotic Eukaryote Eukaryote
1. internal membrane bound organelles with specific structures and functions. with a. cell membrane surrounding cell cytoplasm cytoplasm b. nuclear membrane around nucleus c. endoplasmic reticulum endoplasmic d. mitochrondria e. chloroplasts PLANT vs. ANIMAL CELLS PLANT
What are the principal differences between Plant and animals cells? Which organelles do they share? Which are unique? Organelles Organelles
Chloroplasts Central vacuole Cell wall AUTOTROPHIC ANIMALS:
No chloroplasts No vacuoles No cell wall HETEROTROPHIC An animal cell An A plant cell plant Organelles Organelles
A. Nucleus A. Nucleus B. Nucleolus B. Nucleolus C. Membrane systems C. Membrane D. Mitochondria D. Mitochondria E. Golgi bodies E. Golgi F. Chloroplasts F. Chloroplasts 1. endoplasmic reticulum G. Central Vacuole 1. endoplasmic G. Central a. smooth ER a. smooth H. Cell wall H. Cell b. rough ER b. rough I. Flagella and cilia Flagella 2. ribosomes 2. ribosomes The nucleus and its envelope Endoplasmic reticulum (ER) Endoplasmic Ribosomes Ribosomes Golgi bodies Golgi The endomembrane system The mitochondrion The Ultrastructure of a eukaryotic flagellum or cilium Ultrastructure The plant cell vacuole The chloroplast The In lab: In
A. Bacterial cells A. Bacterial B. Human epidermal cells B. Human stain- methylene blue C. Onion epidermis C. Onion D. Hydrilla Hydrilla Use glass slides throughout lab, then throw them in SHARPS container. SHARPS Microscope and Cell Structure Data sheet (10 pts.) Data
A. Complete the data sheet on pp. 9-10. B. THIS IS DUE BEFORE YOU LEAVE THIS THE LAB. THE The "protists” The
A. Representing at least 5 kingdoms A. Representing B. 60,000 extant species B. 60,000 C. Eukaryotic cells C. Eukaryotic origin from prokaryotes by origin endosymbiosis endosymbiosis MANY PROTIST CULTURES INCLUDE Euglena AS FOOD. Euglena THESE CULTURES ARE NOT CONTAMINATED. NOT This week we will include:
A. Kingdom Euglenozoa 1. Phylum Euglenophyta - Euglena Phylum Euglena 2. Phylum Kinetoplastida – Phylum Trichonympha, Trypanosoma Trichonympha, B. Kingdom Alveolata B. Kingdom Phylum Ciliophora - Paramecium Paramecium C. Kingdom Amoebozoa C. Kingdom Phylum Gymnamoeba - Amoeba Amoeba (pp.85-86) (pp.85-86) Euglena is a genus of unicellular Euglena protists, of the class Euglenoidea of the phylum Euglenozoa (also known as Euglenophyta). Termite and Trichonympha Trichonympha Trichonympha is a genus of Trichonympha parabasalian protists that live in the intestines of many, if not most, termite species. They are symbiotes, in that they break down the cellulose in the wood and plant fibers their hosts eat. and Trypanosoma, the kinetoplastid that causes sleeping sickness Trypanosoma Chagas disease Conjugation and genetic recombination in Paramecium caudatum Paramecium Paramecium conjugating Paramecium Amoeba proteus Amoeba The Amoebozoa are a major group of amoeboid protozoa, The Amoebozoa including the majority that move by means of internal cytoplasmic flow, called pseudopodia flow, Paramecium contractile Paramecium vacuole EXERCISE vacuole
A. B. Pp. 81-83. You may find this exercise to be difficult. It is You meant to encourage you to develop your skills with the microscope. the C. Each of you must count the number of contractions Each in 2 minutes for one Paramecium in fresh. in D. Then we will combine our data for the class. E. Paramecium contractile vacuole data sheet (5 pts.). Due at end of the lab. Paramecium contractile Paramecium vacuole EXERCISE vacuole
movie Do the Enzyme Kinetics Prelab (pp. 55-56) before next lab. (pp. Next Lab you have Lab practical Readings: Chapter 5 and Readings: Appendix A Appendix ...
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This note was uploaded on 04/24/2011 for the course BSC 2010L taught by Professor Herrerabaerbolker during the Spring '08 term at University of Florida.
- Spring '08