Enzymes - Turninyourhomework BSC 2010L ENZYME KINETICS In...

Info iconThis preview shows pages 1–22. Sign up to view the full content.

View Full Document Right Arrow Icon
Turn in your homework
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
BSC 2010L - ENZYME KINETICS In this lab, we will study the influence of temperature and pH on the rate of an enzymatic reaction using spectrophotometry.
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Spectrophotometry Review
Background image of page 10
Proteins A protein's function is due entirely to its overall shape = conformation. Conformation is determined by 1˚, 2˚, 3˚, and perhaps 4˚ structure of the protein.
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Primary Structure
Background image of page 12
Secondary structure
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Tertiary structure
Background image of page 14
Quaternary structure
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Enzymes A. Enzymes are protein catalysts. B. Lower the activation energy necessary for a chemical reaction to occur. C. Act on a substrate , form an enzyme- substrate complex . Ultimately results in a product . D. We are using barley amylase enzyme on a starch substrate.
Background image of page 16
Background image of page 17

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Formation of an enzyme-substrate complex
Background image of page 18
Environmental factors affecting enzyme activity
Background image of page 19

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Experiments: A. Work in groups of two to four. B. You will perform one run from the pH experiment or one from temperature experiment.
Background image of page 20
BASIC PROCEDURE A. Set spectrophotometers to the λ max we determined for the starch/I 2 KI solution (560 nm). B. In an erlenmeyer, add 35 ml starch + 35 ml H
Background image of page 21

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 22
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/24/2011 for the course BSC 2010L taught by Professor Herrerabaerbolker during the Spring '08 term at University of Florida.

Page1 / 34

Enzymes - Turninyourhomework BSC 2010L ENZYME KINETICS In...

This preview shows document pages 1 - 22. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online