LabTech(2) - BSC 2010L BSC The Scientific Method & The...

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Unformatted text preview: BSC 2010L BSC The Scientific Method & The Lab Techniques Lab Jackson Frechette jluc@ufl.edu jluc@ufl.edu Administrative matters: Administrative A. Lab syllabus B. Lab points breakdown sheet C. Lab policies (w/syllabus) - Briefly C. review. review. D. Lab Safety (w/syllabus) In this lab we will: In A. Go over course organization. B. Discuss volumetric glassware. B. C. Discuss the Scientific Method. C. D. Describe use of the spectrophotometer. D. E. Do a graphing exercise. E. F. Collect data for an absorption spectrum of F. a starch/iodine solution. starch/iodine A Scientific Method of Investigation Hypothesis Testing Hypothesis A. Idealized pattern of investigation: 1. 2. 3. 4. 5. Observation Questioning (Inductive reasoning) Hypothesis Prediction (Deductive reasoning) Experimentation a. Observation b. Experimentation 6. Data collection and analysis 7. Re-evaluation of hypothesis The Movie Theater Scenario The 1. Suppose you are an observer in a movie theater. A woman comes in and sits Suppose down. Five minutes later, a man comes in and sits down next to her without saying a word. saying These are your observations. What questions might you ask of this situation? These (You cannot talk with the subjects.) (You Make an hypothesis. Make Make a prediction. (Think of these as "If, then" statements.) Conduct experiments. Conduct Re-evaluate. Re-evaluate. Are there alternative hypotheses that can be imagined for which the same Are observations would hold? 2. 3. 4. 4. 5. 6. 6. 7. 7. Scientific Method exercise Scientific 1. Complete the exercise on pp. 25-26 of your manual (5 pts.) at home and hand in next lab. Volumetric glassware Volumetric A. Glass pipettes, graduated cylinders, A. ehrlenmeyer flasks and graduated beakers. beakers. B. Micropipetter. Exercise: practice loading gels Exercise: for electrophoresis. for Graphing of Biological Data Graphing Cricket Chirp exercise Cricket 1. Complete the graphing exercise on pp. 37 and 38 of your manual. 2. Turn this in to me when you are finished. Remember, do this work by yourself. Picture 2 21 20 19 Chirp Rate (Hz) 18 17 16 15 14 20 25 30 35 Temperature ( C) Figure 1. Chirp frequency (Hz) of the striped ground cricket ( Nemobius allardi ) at different ambient air temperatures. The data presented are those of Pierce (1949). Spectrophotometry Spectrophotometry A. Use of spectrophotometers. http://www.youtube.com/watch?v=V1vXCmhWw40 B. Zero the machine. B. C. Composition and use of a blank. D. How to adjust wavelength. E. Proper use of cuvettes. Spec exercise Spec A. Students work in pairs. B. Use micro- and macropipetters in preparing cuvettes. C. Blank : 8 mls water + 0.1 ml I2KI. C. Blank D. Experimental: 4 ml starch: 4 ml water: 0.1 ml I2KI. D. Experimental E. Measure absorbance from 440 - 680 nm in 40 nm E. 440 40 increments. Record in table of p. 39. Record F. Reblank the machines after each change of F. wavelength. wavelength. G. Mix cuvette by inverting before taking a reading. Absorption Spectrum Absorption A. Laboratory Skills and Techniques sheet (p. 313) A. WHAT SKILLS CAN YOU LIST FROM THIS LAB? B. There will be a quiz at the beginning of the next lab. ...
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This note was uploaded on 04/24/2011 for the course BSC 2010L taught by Professor Herrerabaerbolker during the Spring '08 term at University of Florida.

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