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Unformatted text preview: BSC 2010L BSC The Scientific Method & The Lab Techniques Lab TA: Iwan Molgo Email: imolgo@ufl.edu Office hours: Mondays 10:40 -12:35 or Office by appointment Dickinson Hall 385 B by In this lab we will: In A. Course organization. B. Introduce yourself C. Volumetric glassware. Volumetric D. The Scientific Method. D. E. Do a graphing exercise. F. Describe use of the spectrophotometer. G. Collect data for an absorption spectrum of Collect a starch/iodine solution. starch/iodine Administrative matters: Administrative A. Lab syllabus B. Lab points breakdown sheet C. Lab policies (lab manual) - Briefly C. review review D. Lab admit policies (HANDOUT) E. Lab Safety (w/syllabus) BSC 2010L Administrative Policies Lab Policies Lab 1. Students are required to attend their officially registered section 1. of lab. Students with valid and DOCUMENTED excuses must notify their TA within 24 hours of the lab start time and subsequently provide written documentation. An alternative time to attend lab will be arranged or appropriate makeup lessons will be assigned. Missed labs must be made up within the same week. Missed If you know in advance that you are unable to make your regular If sections, arrange to attend a lab section earlier in the week. sections, Students without documented excuses may not attend makeup labs. Students They will be allowed to makeup work with grade penalties. 2. If you are ill, do not come to lab. Notify your TA immediately. Get documentation of your illness from a doctor or the infirmary. Get Your instructor has the right to ask you to leave the classroom if Your you appear to be ill. you Lab Policies (cont.) Lab 3. Data sheets and other graded assignments are due 3. either in class or at the beginning of the next lab beginning session. session. Assignments turned in after the start of the lab will be Assignments considered late. considered If you are unable to turn in your work during your regular lab If section and are not able to hand it in directly to your instructor, DO NOT leave an assignment at your instructor's office. Rather, instructor's (1) make a photocopy of your assignment for safekeeping and (2) hand in the original to the staff of the Biological Sciences office (2) (220 Bartram Hall) between 8 a.m. and 4 p.m. between Lab Policies (cont.) Lab 4. Late work will be penalized 10% of the total points per day. day 5. Students may be allowed to turn in assignments from 5. makeup assignments or on which they did not participate in the collection of data at the discretion of the lab coordinator. These will be penalized at least the 20%. 20%. 6. No food or drink is permitted in the labs. 6. No Students are expected to leave the lab in a clean and orderly state. and 7. Students are not allowed to wear sandals or opentoed shoes in the laboratories. Lab Policies (cont.) Lab 8. Students are expected to do their own work. All data 8. Students All sheets and lab reports are to be written individually and without plagiarism from the lab manual, the text, assignments from previous semesters, or any other source. Experiments done by groups must be written up as though the individual has done all parts of the experiment personally. Any evidence of copying or working together will result in the loss of all points on that graded assignment and the names of those students s0 penalized will be reported to the university's office of student judicial affairs. student Lab Safety Lab Work in the Biology laboratory may expose students to inherently dangerous activities. Work inherently activities 1. 2. 3. 4. 4. 5. Smoking, eating, and drinking are expressly forbidden and NOT allowed in the laboratory. Locate the placement of safety equipment and supplies in the laboratory. Students should follow instructions carefully, especially when hazardous conditions occur or hazardous Students materials are being used. Students should dress appropriately in the lab. Gloves and protective aprons will be made available in the labs. Students Students may elect to supply their own gloves and protective aprons or laboratory coats. Only shoes that provide complete foot covering are allowed in the lab. provide You should be familiar with fire procedures. Leave the building immediately should a major fire occur or if the You fire alarm sounds. Notify the appropriate authorities -- don't assume someone else remembered to do it. Meet with other students and your instructor outside the building before leaving so that an accurate headcount may be made. The safe use of specific equipment and tools (e.g., microscopes, slides, scalpels, and pipettes) will be The demonstrated by the instructor during the laboratory sessions. Be sure you understand this usage and assk questions if you do not. Never pipet by mouth. Always use a suction bulb or pipet aid. Notify your T.A. IMMEDIATELY of any spills, breakages, or equipment malfunction. Students should report all hazardous conditions to the instructor immediately. Students All organisms, living or dead, should be treated with care and respect. Avoid direct handling when possible. All Students should clean up any supplies used and should return materials where they belong as instructed. Any Students material spilled should be cleaned appropriately. Report any hazardous spills or breakages. Broken glass and sharp metal waste should be placed only in those receptacles marked for such disposal -- do Broken not put these materials in normal trash receptacles. Work areas must be left clean and dry prior to leaving the lab. Chemicals and reagents must be returned to their Work proper places. You should always wash your hands before leaving the laboratory, even if you have not knowingly come in You contact with any chemicals or biological fluids. 6. 6. 7. 7. 8. 9. 10. 10. 11. 11. 12. 12. 13. 13. 14. 14. Your final letter grade will be based on percentage of total points earned: points A ≥ 90.0% A- ≥86.7% B+≥ 83.3% B ≥ 80.0% B- ≥ 76.7% C+≥ 73.3% C ≥ 70.0% C- ≥ 66.7% Introduce yourself to the class Introduce A Scientific Method of Investigation Hypothesis Testing Hypothesis A. Idealized pattern of investigation: 1. 2. 3. 4. 5. Observation Questioning (Inductive reasoning) Hypothesis Prediction (Deductive reasoning) Experimentation a. Observation b. Experimentation 6. Data collection and analysis 7. Re-evaluation of hypothesis Obser vati ons of a Ophr ys (or chi d) habi tat OBS 1 OBS OBS 2 OBS Idealized pattern of investigation: Idealized 1. Observation 2. Questioning (Inductive reasoning) 3. Hypothesis 4. Prediction (Deductive reasoning) 5. Experimentation a. Observation b. Experimentation 6. Data collection and analysis 7. Re-evaluation of hypothesis The Movie Theater Scenario The 1. Suppose you are an observer in a movie theater. A woman comes in and sits Suppose down. Five minutes later, a man comes in and sits down next to her without saying a word. saying These are your observations. What questions might you ask of this situation? These (You cannot talk with the subjects.) (You Make an hypothesis. Make Make a prediction. (Think of these as "If, then" statements.) Conduct experiments. Conduct Re-evaluate. Re-evaluate. Are there alternative hypotheses that can be imagined for which the same Are observations would hold? 2. 3. 4. 4. 5. 6. 6. 7. 7. Scientific Method exercise Scientific Complete the exercise on p. 25 & 26 of your manual (5 pts.) Volumetric glassware Volumetric A. Glass pipettes, graduated cylinders, A. ehrlenmeyer flasks and graduated beakers. beakers. B. Micropipetter. Gr aduated Beaker s Gr Erlenmeyer flasks Erlenmeyer Gr aduated Gr Cyl i nder s A B Gr aduated pi pettes Gr Whi ch one i s pr eci sel y on 13.6 ml ? Whi M i cr o Pi pette Graphing of Biological Data Graphing Go to Go http://www.bsc.ufl.edu/ http://www.bsc.ufl.edu/ Login to BioViewer Password is: anatomy Click on ‘How to build a figure I n al l your assi gnments, I w ant you to wr i te t wo ti tl es: A br i ef one above the f i gur e and a d etai l ed d escr i pti on bel ow the fi gur e bel ( Cobur n et a l , 2009) (Cobur et 2009) Cricket Chirp exercise Cricket 1. Complete the graphing exercise on pp. 37 and 38 of your manual. 2. Turn this in to me when you are finished. (5 pts.) Remember, do this work by yourself. 21 Chirp Rate (Hz) 20 19 18 17 16 15 14 20 25 30 35 Temperature ( C) Figure 1. Chirp frequency (Hz) of the striped ground cricket (Nemobius allardi ) at different ambient air temperatures. The data presented are those of Pierce (1949). Chirp Rate (Hz) 21 20 19 18 17 16 15 14 20 25 30 35 Temperature ( C) Figure 1. Chirp frequency (Hz) of the striped ground cricket ( Nemobius allardi ) at different ambient air temperatures. The data presented are those of Pierce (1949). Spectrophotometry Spectrophotometry A. Use of spectrophotometers. http://www.youtube.com/watch?v=V1vXCmhWw40 B. Zero the machine. B. C. Composition and use of a blank. D. How to adjust wavelength. E. Proper use of cuvettes. Spec exercise Spec A. Students work in pairs. B. Use micro- and macropipetters in preparing cuvettes. C. Blank : 8 mls water + 0.1 ml I2KI. C. Blank D. Experimental: 4 ml starch: 4 ml water: 0.1 ml I2KI. D. Experimental E. Measure absorbance from 440 - 680 nm in 40 nm E. 440 40 increments. Record in table of p. 39. Record F. Reblank the machines after each change of F. wavelength. wavelength. G. Mix cuvette by inverting before taking a reading. Absorption Spectrum Absorption N ext L ab • Office hours: Mondays 10:40 -12:35 or by appointment Dickinson Hall 385 B Next week : no Lab • Next lab – Reading Assignment: Chapters 1, 2, 7 and 8 (p. 85 – 86) – QUIZ 1 – based on this week’s lab and on the reading material for next lab ...
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