popgen2invert1 - BSC 2010L POPULATION GENETICS II &...

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Unformatted text preview: BSC 2010L POPULATION GENETICS II & INVERTEBRATE DIVERSITY I I In this lab, we will: A. Complete the Polymerase Chain Reaction (PCR) exercise. B. Collect data for the Hardy-Weinberg analysis. C. Begin our study of invertebrate diversity . . PCR Exercise - Second Week Procedures Get you PCR sample and vortex it Then, pipette 7 l into a well in the gel. Be sure to record the loading order of the samples. Each gel should also have at least one lane with the 7 l of the Pgem ladder. We will run the gels at 120 V for 30 min. Then we will photograph the gels on the UV transilluminator. Finally, you will score the gels. The band with the insert will run slower than the band without the insert. Is our population in Hardy-Weinberg Equilibrium for the Alu insert? Alu insert missing Alu insert (300bp) primer primer primer primer 100 bp 400 bp Alu Insert How to read the final gels : : These data are used to complete the Hardy-Weinberg Equilibrium data sheet(p183) (10 pts.) This is due at the end of lab. In addition, they must write an abstract of this PCR exercise (10 pts.). This will be due at the next lab. Assumptions of Hardy-Weinberg Equilibrium Conditions for genetic equilibrium: a. random mating b. very large population = no genetic drift c. no selection: all genotypes are equally viable d. no mutation e. no migration = no gene flow H/W Equation H/W equation predicts the number of individuals of each genotype if the population is in equilibrium Need to know the allele frequencies in the population Plug those allele frequencies into the equation to get the expected genotype frequencies for a population in equilibrium Compare those predicted genotype frequencies to the observed genotype frequencies Remember: gene frequencies of a particular generation depend upon the gene frequencies of the previous generation and not upon the genotype frequencies Students frequently use genotype and allele frequency interchangeably in lab reports, so stress the difference How do we get allele frequencies? If you know the genotype of all the individuals in the population, then you can calculate the allele frequencies For example: 25 individuals are AA 17 are AB 30 are BB What is the allele frequency of A? What is the allele frequency of B? Calculating Allele Frequencies (p 177) Genotype AA AB BB # of individuals 25 17 30 # A __ __ __ # B __ __ __ Totals: ___ ___ ___ A allele frequency = p = A / (# of individuals x 2) B allele frequency = q = B / (# of individuals x 2) To relate allele frequencies to genotypic...
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This note was uploaded on 04/24/2011 for the course BSC 2010L taught by Professor Herrerabaerbolker during the Spring '08 term at University of Florida.

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popgen2invert1 - BSC 2010L POPULATION GENETICS II &...

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