2A-A16-SPEC-NGUYEN,VU

2A-A16-SPEC-NGUYEN,VU - Thymol Blue Absorbance @ 434 nm...

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Unformatted text preview: Thymol Blue Absorbance @ 434 nm -> Bromothymol Blue Absorbance @434 nm -> Thymol Blue Solution At Bromothymol Blue Maxiumum Absorption (6 0.14 0.12 0.1 Absornace Absorbance 0.08 0.06 0.04 0.02 0 0 10 20 30 40 50 60 70 Concentration(Micromolar) f(x) = 0x + 0.01 R² = 0.98 Absorbance Linear Regression for Absorbance Bromothymol Blue Solution At Thymol Blue Maxiumum Absorption (4 0.14 0.12 0.1 0.08 0.06 0.04 0.02 0 0 5 10 15 20 25 Concentration (Micromolar) f(x) = 0.01x + 0 R² = 0.95 Column E Linear Regression for Column E 0.04 0.02 0 0 5 10 15 20 25 Concentration (Micromolar) e Molarity Absorbance Thymol Blue Absorbance @ 618nm -> Molarity 61.6 0.79 61.6 49.3 0.63 49.3 36.9 0.47 36.9 24.6 0.32 24.6 12.3 0.16 12.3 Molarity Absorbance Bromothymol Blue Absorbance @618 nm -> Molarity 23.2 0.12 23.2 18.56 0.1 18.56 13.92 0.09 13.92 9.28 0.05 9.28 4.64 0.02 4.64 Thymol Absorption (618nm) Blue Solution At Thymol Blue Maxiumum Absorpt 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 10 20 30 40 50 60 70 Concentration (Micromolar) Absorbance orbance ear Regression for orbance f(x) = 0.01x + 0 R² = 1 Absorba Linear R Absorba Bromothymol bsorption (434nm) Blue Solution At Bromothymol Blue Maximum Ab 0.8 0.7 0.6 umn E ar Regression for umn E 0.5 0.4 0.3 0.2 0.1 0 0 5 10 15 20 25 Concentration (Micromolar) Absorbance f(x) = 0.03x + 0 R² = 1 Colum Linear Colum 0.2 0.1 0 0 5 10 15 20 25 Concentration (Micromolar) e Absorbance 0.13 0.1 0.09 0.05 0.03 Absorbance 0.67 0.52 0.38 0.27 0.14 axiumum Absorption (434nm) Absorbance Linear Regression for Absorbance 60 70 Blue Maximum Absorption (618nm) Column H Linear Regression for Column H 20 25 20 25 wavelength BB 370 380 390 400 410 420 430 434 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 618 620 630 640 650 660 670 TB 0.16 0.18 0.2 0.2 0.19 0.16 0.13 0.12 0.11 0.1 0.1 0.1 0.1 0.12 0.13 0.16 0.18 0.22 0.26 0.31 0.16 0.43 0.49 0.56 0.63 0.67 0.69 0.67 0.67 0.49 0.03 0.21 0.12 0.5 0.55 0.6 0.67 0.72 0.77 0.79 0.79 0.78 0.75 0.68 0.6 0.52 0.42 0.34 0.27 0.22 0.18 0.16 0.15 0.15 0.16 0.17 0.18 0.18 0.15 0.13 0.11 0.09 0.05 0.03 0.02 0.01 sum 0.66 0.73 0.8 0.87 0.91 0.93 0.92 0.91 0.89 0.85 0.78 0.7 0.62 0.54 0.47 0.42 0.4 0.4 0.42 0.46 0.31 0.58 0.66 0.74 0.8 0.81 0.82 0.79 0.76 0.54 0.06 0.23 0.13 Unknown BTB and TB 0.28 0.31 0.35 0.37 0.39 0.39 0.38 0.38 0.37 0.35 0.32 0.29 0.26 0.23 0.2 0.19 0.18 0.18 0.2 0.22 0.25 0.28 0.32 0.36 0.4 0.42 0.42 0.42 0.37 0.29 0.19 0.12 0.09 (TB+BTB) & U 1 0.9 0.8 0.7 0.6 Absorbance 0.5 0.4 0.3 0.2 0.1 0 350 400 450 Bromothymol Blue, Thymol Blue, SUM (TB+BTB) & Unknown BTB and TB SCANS at Wavelengths 370-670 450 500 Wavelength (nm) 550 600 650 hs 370-670 BB TB sum Unknown BTB and TB 650 700 ...
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This note was uploaded on 04/25/2011 for the course CHEM 2A taught by Professor Guo during the Spring '08 term at UC Davis.

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