expt 4 - spectrophotometer

expt 4 - spectrophotometer - CHM171L Physical Chemistry 2...

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CHM171L Physical Chemistry 2 Laboratory 1 st Quarter SY 2010-2011 Spectrophotometry Calderon, Edna 1 , Mangundayao, Ace P., Marquez, Victoria Marie A., Mateo, Neechelle Ann M. 2 1 Professor, School of Chemical Engineering, Chemistry and Biotechnology, Mapua Institute of Technology; 2 Student (s), Subject/Section, School of Chemical Engineering, Chemistry and Biotechnology, Mapua Institute of Technology ABSTRACT A spectrophotometer is employed to measure the amount of light that a sample absorbs. The instrument operates by passing a beam of light through a sample and measuring the intensity of light reaching a detector. In this experiment we were able to determine the color that was absorbed and reflected by the given amount of wavelength. We were also asked to determine the absorption of spectrum of Cobalt (II) Nitrate solution with varying wavelengths from 375 to 800. And last, the beer’s law plot to determine the concentration of the unknown solution at a given absorbance. With the use of computer software, we were able to determine the properties needed like absorbance and concentration. Keywords : spectrophotometer, wavelength, Cobalt(II) Nitrate Solution, beer’s law plot INTRODUCTION Spectrophotometer measures the amount of photon absorbed after it passes through a solution sample. It consists of two types of spectrophotometry: 1. ultraviolet-visible - uses light in the ultraviolet range (185-400 nm) and visible range (400-700 nm) of electromagnetic radiation spectrum 2. infrared - uses light in the infrared range ( of 700-15000 nm) of electromagnetic radiation spectrum These spectrophotometers consist of two devices: 1. spectrometer - a device that produces, typically disperses and measure light A spectrometer produces a light beam (photon) that will go through a prism to create desired wavelengths of light. After the light beam passes through the solution of a sample, the photometer will detect the amount of photon that is absorbed and send a signal to a galvanometer. 2. photometer - a device that measures the
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This note was uploaded on 04/27/2011 for the course CHM 171L taught by Professor Ng during the Spring '11 term at Mapúa Institute of Technology.

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expt 4 - spectrophotometer - CHM171L Physical Chemistry 2...

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