EEMB_3L_Review_-_Gail_Drus_

EEMB_3L_Review_-_Gail_Drus_ - Prokaryotes Shapes Gram...

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Unformatted text preview: Prokaryotes Shapes Gram staining RFLP Analysis Add Primers (16s) CR PCR Restriction digestion (Hae III) Gel electrophoresis Eubacteria Arabinose testing Electrophoresis Electrophoresis Lanes were loaded (from left to right): Lanes were loaded (from left to right): 1: 1: Stds Stds 2: Uncut unknown 2 2: Uncut unknown 2 anes 3 anes 3 - : Unknowns 1 : Unknowns 1- , 16S RNA Lanes 3 Lanes 3 11: Unknowns 1 11: Unknowns 1 9, 9, Lane 12: Uncut unknown 4 Lane 12: Uncut unknown 4 Lane Band Size 1 Size Standards 677, 517, 452, 395, 353, 223, 178, 125 2 Uncut E. aerogenes (U2) 548 3 B. subtilis 365, 225 4 E. Aerogenes U2) 241, 191, 136 g ( ) , , 5 Enterococcus faecalis (U3) 387, 261 6 Streptomyces griseus (U4) 276, 131, 111 7 E. coli (U5) 257, 206, 156, 139 8 Bacillus megaterium (U1, U6) 285, 243, 99 9 Micrococcus luteus (U7) 287, 132, 115, 66 10 Pseudomonas flavescens (U8) 407, 201 11 Micrococcus roseus 280, 124, 106 Unknown IDs Unknown IDs 12 Uncut B. subtilis 535 Cyanobacteria (Eubacteria)...
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EEMB_3L_Review_-_Gail_Drus_ - Prokaryotes Shapes Gram...

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