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Unformatted text preview: other using the Gabriel sequence to accomplish the task. 4. Supply reagents that could be used to accomplish the sequence illustrated below. CH 2 (CO 2 Et) 2 CO 2 Et CO 2 Et CO 2 H 5. Given a pH of 7.3, determine the net charge on aspartic acid, histidine, arginine. Youre your work. Would it be possible to separate these substances using gel electrophoresis (at this pH)? Explain. 6. Determine the pI for asparagine. Show your reasoning in the manner illustrated in todays class. 7. Determine the equilibrium constant for the acid-base reaction of diethyl malonate and sodium ethoxide. Show your work. [Hint: You will have to consult Appendix II of your text to determine the appropriate pKa values for the component acids; use values that are rounded-off.] Generalize, in words, what you have discovered....
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- Spring '09